R-Spondin Monoclonal Antibody
- 货号：YM0566
- 应用：WB;ELISA
- 种属：Human
- 简介：
- >>Wnt signaling pathway
- 免疫原：
- Purified recombinant fragment of R-spondin1 expressed in E. Coli.
- 特异性：
- R-Spondin Monoclonal Antibody detects endogenous levels of R-Spondin protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- RSPO1;R-spondin-1;Roof plate-specific spondin-1;hRspo1
- 背景：
- This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014],
- 功能：
- disease:Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC) [MIM:610644]. This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin.,domain:The FU repeats are required for activation and stabilization of beta-catenin.,function:Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.,miscellaneous
- 细胞定位：
- Secreted . Nucleus . Seems to mainly localize to nucleoli. .
- 组织表达：
- Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen.
- Western Blot analysis using R-Spondin Monoclonal Antibody against recombinant R-spondin1 protein (1) and R-spondin1(aa21-263)-hIgGFc transfected HEK293 cell lysate(2).
