TORC3 Monoclonal Antibody
- 货号:YM0626
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Monkey
- 简介:
- >>Human T-cell leukemia virus 1 infection
- 蛋白名称:
- CREB-regulated transcription coactivator 3
- 免疫原:
- Purified recombinant fragment of human TORC3 expressed in E. Coli.
- 特异性:
- TORC3 Monoclonal Antibody detects endogenous levels of TORC3 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释:
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- Affinity purification
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CRTC3;TORC3;CREB-regulated transcription coactivator 3;Transducer of regulated cAMP response element-binding protein 3;TORC-3;Transducer of CREB protein 3
- 背景:
- This gene is a member of the CREB regulated transcription coactivator gene family. This family regulates CREB-dependent gene transcription in a phosphorylation-independent manner and may be selective for cAMP-responsive genes. The protein encoded by this gene may induce mitochondrial biogenesis and attenuate catecholamine signaling in adipose tissue. A translocation event between this gene and Notch coactivator mastermind-like gene 2, which results in a fusion protein, has been reported in mucoepidermoid carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012],
- 功能:
- function:Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).,similarity:Belongs to the TORC family.,subcellular location:Appears to be mainly nuclear.,subunit:Binding, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1 enhances recruitment of TAF4 to the promoter. 'Arg-314' in the bZIP domai
- 细胞定位:
- Nucleus . Cytoplasm . Appears to be mainly nuclear (PubMed:15454081). Translocates to the nucleus following adenylyl cyclase or MAP kinase activation (PubMed:30611118). .
- 组织表达:
- Predominantly expressed in B and T lymphocytes. Highest levels in lung. Also expressed in brain, colon, heart, kidney, ovary, and prostate. Weak expression in liver, pancreas, muscle, small intestine, spleen and stomach.
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- Western Blot analysis using TORC3 Monoclonal Antibody against HeLa (1), Jurkat (2), Cos7 (3) and MCF-7 (4) cell lysate.
![](/uploadfiles/PIC/ym0626-ihc.jpg)
- Immunohistochemistry analysis of paraffin-embedded breast cancer (left) and ovarian cancer (right) with DAB staining using TORC3 Monoclonal Antibody.
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- Immunofluorescence analysis of NTERA-2 cells using TORC3 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.