G3BP1 (PTR1324) mouse mAb
- 货号:YM4539
- 应用:WB;IF;ELISA
- 种属:Human;Mouse;Rat;
- 蛋白名称:
- Ras GTPase-activating protein-binding protein 1 (G3BP-1) (EC 3.6.4.12) (EC 3.6.4.13) (ATP-dependent DNA helicase VIII) (hDH VIII) (GAP SH3 domain-binding protein 1)
- 免疫原:
- Synthesized peptide derived from human protein. AA range: 200-300
- 特异性:
- This antibody detects endogenous levels of G3BP1 protein.
- 组成:
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- 来源:
- Mouse, Monoclonal/IgG2b, kappa
- 稀释:
- WB 1:500-2000. IF 1:100-500. ELISA 1:1000-5000
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- Ras GTPase-activating protein-binding protein 1 (G3BP-1) (EC 3.6.4.12) (EC 3.6.4.13) (ATP-dependent DNA helicase VIII) (hDH VIII) (GAP SH3 domain-binding protein 1)
- 背景:
- G3BP stress granule assembly factor 1(G3BP1) Homo sapiens This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008],
- 功能:
- ATP- and magnesium-dependent helicase that plays an essential role in innate immunity . Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS. Enhances also DDX58-induced type I interferon production probably by helping DDX58 at sensing pathogenic RNA . In addition, plays an essential role in stress granule formation . Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends . Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency . Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA . Phosphorylation-dependent sequence-specific endoribonuclease in vitro . Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR .
![](/uploadfiles/PIC/PTR1324-Jurkat.jpg)
- Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-G3BP-1 (PTR1324) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody.
Lane 1: Jurkat
Lane 2: HEK293
Lane 3: Hela