HLA-DOα Polyclonal Antibody
- 货号：YT2177
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 简介：
- >>Phagosome;>>Cell adhesion molecules;>>Antigen processing and presentation;>>Hematopoietic cell lineage;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>Intestinal immune network for IgA production;>>Type I diabetes mellitus;>>Leishmaniasis;>>Toxoplasmosis;>>Staphylococcus aureus infection;>>Tuberculosis;>>Influenza A;>>Human T-cell leukemia virus 1 infection;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Asthma;>>Autoimmune thyroid disease;>>Inflammatory bowel disease;>>Systemic lupus erythematosus;>>Rheumatoid arthritis;>>Allograft rejection;>>Graft-versus-host disease;>>Viral myocarditis
- 蛋白名称：
- HLA class II histocompatibility antigen DO alpha chain
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human HLA-DOA. AA range:71-120
- 特异性：
- HLA-DOα Polyclonal Antibody detects endogenous levels of HLA-DOα protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- HLA-DOA;HLA-DNA;HLA-DZA;HLA class II histocompatibility antigen; DO alpha chain;MHC DN-alpha;MHC DZ alpha;MHC class II antigen DOA
- 背景：
- HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. [provided by RefSeq, Jul 2008],
- 功能：
- function:Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule.,polymorphism:The only allele of DOA known is DOA*0101 which is shown here.,similarity:Belongs to the MHC class II family.,similarity:Contains 1 Ig-like C1-type (immunoglobulin-like) domain.,subunit:Heterodimer of an alpha chain (DOA) and a beta chain (DOB).,
- 细胞定位：
- Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Complexes with HLA-DM molecule during intracellular transport and in endosomal/lysosomal compartments. Heterotetramerization is necessary to exit the ER.
- Western Blot analysis of various cells using HLA-DOα Polyclonal Antibody
- Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from COLO cells, using HLA-DOA Antibody. The lane on the right is blocked with the synthesized peptide.
