IKKα/β Polyclonal Antibody

    • 货号:YT2302
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat;Pig
      • 靶点:
      • IKKα/β
      • 简介:
      • >>Antifolate resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>Chemokine signaling pathway;>>NF-kappa B signaling pathway;>>FoxO signaling pathway;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Osteoclast differentiation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>Cytosolic DNA-sensing pathway;>>C-type lectin receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>TNF signaling pathway;>>Adipocytokine signaling pathway;>>Alcoholic liver disease;>>Alzheimer disease;>>Epithelial cell signaling in Helicobacter pylori infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Yersinia infection;>>Chagas disease;>>Toxoplasmosis;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Human pap
      • 基因名称:
      • CHUK/IKBKB
      • 蛋白名称:
      • Inhibitor of nuclear factor kappa-B kinase subunit alpha
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta. AA range:141-190
      • 特异性:
      • IKKα/β Polyclonal Antibody detects endogenous levels of IKKα/β protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • CHUK;IKKA;TCF16;Inhibitor of nuclear factor kappa-B kinase subunit alpha;I-kappa-B kinase alpha;IKK-A;IKK-alpha;IkBKA;IkappaB kinase;Conserved helix-loop-helix ubiquitous kinase;I-kappa-B kinase 1;IKK1;Nuclear factor NF-kappa-B
      • 实测条带:
      • 85kD
      • 背景:
      • This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],
      • 功能:
      • catalytic activity:ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].,enzyme regulation:Activated when phosphorylated and inactivated when dephosphorylated.,function:Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.,PTM:Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK
      • 细胞定位:
      • Cytoplasm . Nucleus . Shuttles between the cytoplasm and the nucleus.
      • 组织表达:
      • Widely expressed.

      Proliferatins suppress lipopolysaccharide-induced inflammation via inhibition of the NF-κB and MAPK signaling pathways. BIOORGANIC CHEMISTRY2022 Jul;124:105810. Mouse RAW264.7 macrophages
      货号:YT2302

      Induction of PGRN by influenza virus inhibits the antiviral immune responses through downregulation of type I interferons signaling. PLoS Pathogens Plos Pathog. 2019 Oct;15(10):e1008062 WB Human HEK293 cell
      货号:YT2302

      Suppression of CDCA3 inhibits prostate cancer progression via NF‑κB/cyclin D1 signaling inactivation and p21 accumulation. ONCOLOGY REPORTS Oncol Rep. 2022 Feb;47(2):1-10 WB Human 1:1000 Prostate cancer tissue,paracancerous tissue.
      货号:YT2302

      β-Conglycinin-Induced Intestinal Porcine Epithelial Cell Damage via the Nuclear Factor κB/Mitogen-Activated Protein Kinase Signaling Pathway. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2019;67(32):9009–9021 WB Pig 1:1000 IPEC-J2 cell
      货号:YT2302

      Peng, Chenglu, et al. "Glycinin-induced porcine IPEC-J2 cells damage via the NF-κB/MAPK signaling pathway." (2020).
      货号:YT2302

      • 产品图片
      • Western Blot analysis of various cells using IKKα/β Polyclonal Antibody
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IKK-alpha/beta Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HepG2 cells, using IKK-alpha/beta Antibody. The lane on the right is blocked with the synthesized peptide.