Neuro D2 Polyclonal Antibody
- 货号:YT3062
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Neurogenic differentiation factor 2
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human NDF2. AA range:61-110
- 特异性:
- Neuro D2 Polyclonal Antibody detects endogenous levels of Neuro D2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- NEUROD2;BHLHA1;NDRF;Neurogenic differentiation factor 2;NeuroD2;Class A basic helix-loop-helix protein 1;bHLHa1;NeuroD-related factor;NDRF
- 背景:
- This gene encodes a member of the neuroD family of neurogenic basic helix-loop-helix (bHLH) proteins. Expression of this gene can induce transcription from neuron-specific promoters, such as the GAP-43 promoter, which contain a specific DNA sequence known as an E-box. The product of the human gene can induce neurogenic differentiation in non-neuronal cells in Xenopus embryos, and is thought to play a role in the determination and maintenance of neuronal cell fates. [provided by RefSeq, Jul 2008],
- 功能:
- function:Appears to mediate neuronal differentiation.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,
![](/uploadfiles/PIC/yt3062-wb.jpg)
- Western Blot analysis of various cells using Neuro D2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
![](/uploadfiles/PIC/yt3062_pic548818522.jpg)
- Western blot analysis of lysates from 293, COLO, and Jurkat cells, using NDF2 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt3062_pic1017818522.jpg)
- Western blot analysis of the lysates from COLO205 cells using NDF2 antibody.
![](/uploadfiles/PIC/79-ihc-biantaotin2207.jpg)
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).