Rab 34 Polyclonal Antibody
- 货号：YT3930
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- Ras-related protein Rab-34
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human RAB34. AA range:206-255
- 特异性：
- Rab 34 Polyclonal Antibody detects endogenous levels of Rab 34 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000.IHC 1:50-200 ELISA: 1:5000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- RAB34;RAB39;RAH;Ras-related protein Rab-34;Ras-related protein Rab-39;Ras-related protein Rah
- 背景：
- This gene encodes a protein belonging to the RAB family of proteins, which are small GTPases involved in protein transport. This family member is a Golgi-bound member of the secretory pathway that is involved in the repositioning of lysosomes and the activation of macropinocytosis. Alternative splicing of this gene results in multiple transcript variants. This gene overlaps and shares exon structure with the nine-amino acid residue-repeats (NARR) gene, which encodes a functionally distinct nucleolar protein from a different reading frame. [provided by RefSeq, Jan 2012],
- 功能：
- function:Protein transport. Involved in the redistribution of lysosomes to the peri-Golgi region.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Associates with the Golgi complex.,subunit:Interacts with RILP.,
- 细胞定位：
- Cytoplasm . Golgi apparatus . Cytoplasmic vesicle, phagosome . Cytoplasmic vesicle, phagosome membrane ; Lipid-anchor ; Cytoplasmic side . Cell projection, cilium . Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211). .
- 组织表达：
- Brain,Embryo,Heart,Mammary gland,Pancreas,Skeletal muscle,
- Western Blot analysis of various cells using Rab 34 Polyclonal Antibody diluted at 1:500
- Western blot analysis of 293T lysis using Rab 34 antibody. Antibody was diluted at 1:500
- Immunohistochemical analysis of paraffin-embedded Human skeletal muscle. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using RAB34 Antibody. The picture on the right is blocked with the synthesized peptide.
