Phospho IRAK-1 (T100) Cell-Based Colorimetric ELISA Kit

  • Catalog No.:KA1109C
  • Applications:ELISA
  • Reactivity:Human;Mouse;Rat
    • Gene Name:
    • IRAK1
    • Human Gene Id:
    • 3654
    • Human Swiss Prot No:
    • P51617
    • Mouse Swiss Prot No:
    • Q62406
    • Storage Stability:
    • 2-8°C/6 months
    • Other Name:
    • Interleukin-1 receptor-associated kinase 1 (IRAK-1) (EC 2.7.11.1)
    • Detection Method:
    • Colorimetric
    • Background:
    • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.,PTM:Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.,similarity:Contains 1 protein kinase domain.,subunit:IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Interacts with IL1RL1. Interacts with IRAK1BP1.,tissue specificity:Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.,
    • Function:
    • regulation of cytokine-mediated signaling pathway, activation of innate immune response, pattern recognition receptor signaling pathway, toll-like receptor signaling pathway, response to molecule of bacterial origin, activation of immune response, positive regulation of immune system process, MyD88-dependent toll-like receptor signaling pathway, immune response-activating signal transduction, innate immune response-activating signal transduction,immune response-regulating signal transduction, protein complex assembly, protein amino acid phosphorylation,phosphorus metabolic process, phosphate metabolic process, anti-apoptosis, cell surface receptor linked signal transduction, enzyme linked receptor protein signaling pathway, transmembrane receptor protein serine/threonine kinase signaling pathway, intracellular signaling cascade, protein kinase cascade, I-kappaB kinase/NF-kappaB cascade, 
    • Subcellular Location:
    • Cytoplasm . Nucleus . Lipid droplet . Translocates to the nucleus when sumoylated. RSAD2/viperin recruits it to the lipid droplet (By similarity). .
    • Expression:
    • Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.