• Target：
• FoxP3

• Fields：
• >>Th17 cell differentiation;>>Inflammatory bowel disease

• Gene Name：
• FOXP3

• Protein Name：
• Forkhead box protein P3

• Human Gene Id：
• 50943

• Human Swiss Prot No：
• Q9BZS1

• Mouse Gene Id：
• 20371

• Mouse Swiss Prot No：
• Q99JB6

• Immunogen：
• Purified recombinant fragment of human FoxP3 expressed in E. Coli.

• Specificity：
• FoxP3 Monoclonal Antibody detects endogenous levels of FoxP3 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• FOXP3;IPEX;JM2;Forkhead box protein P3;Scurfin

• Molecular Weight(Da)：
• 47kD

• References：
• 1. Roncador G et al. Eur J Immunol. 2005. 35:1681-1691.
  2. Yisong YW. PNAS. 2005 102 (14): 5126-5131.

• Background：
• The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],

• Function：
• disease:Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.,function:Probable transcription factor. Plays a critical role in the control of immune response.,online information:FOXP3 entry,online information:FOXP3 mutation db,similarity:Contains 1 C2H2-type zinc finger.,similarity:Contains 1 fork-head DNA-binding domain.,

• Subcellular Location：
• Nucleus . Cytoplasm . Predominantly expressed in the cytoplasm in activated conventional T-cells whereas predominantly expressed in the nucleus in regulatory T-cells (Treg). The 41 kDa form derived by proteolytic processing is found exclusively in the chromatin fraction of activated Treg cells (By similarity). .

KDM3A promotes inhibitory cytokines secretion by participating in TLR4 regulation of Foxp3 transcription in lung adenocarcinoma cells. Oncology Letters Oncol Lett. 2017 May;13(5):3529-3537 WB,IF Human 1:1000,1:500 A549 cell

Hydroxychloroquine protects against autoimmune premature ovarian insufficiency by modulating the Treg/Th17 cell ratio in BALB/c mice AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY Liping Wang WB Mouse ovaries Treg/Th17 cell

Lactobacillus rhamnosus GG alleviates radiation-induced intestinal injury by modulating intestinal immunity and remodeling gut microbiota MICROBIOLOGICAL RESEARCH Li-Li Zhang IF Mouse 1:100 jejunum tissue

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