VSV-G-Tag Monoclonal Antibody(8D6)

    • Catalog No.:YM3006
    • Applications:WB,IP,IF/ICC
    • Reactivity:Species independent
      • Immunogen:
      • Synthetic Peptide of VSV-G-Tag
      • Specificity:
      • The antibody detects C-terminal, internal, and N-terminal VSV-G fusion proteins.
      • Formulation:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • WB: 1:5000 IP: 1:200 IF: 1:1000
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Storage Stability:
      • -20°C/1 year
      • Background:
      • The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. The VSV-G epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods.
      • Products Images
      • Western blot analysis of VSV-G-TAG protein, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920 was diluted at 1:10000, 37° 1hour.
      • 1ug VSV-G fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000
      • IF analysis of 293T cells transfected with a VSV-G-tagged protein,1:2000 dilution (blue DAPI, red anti-VSV-G)
      • IP antibody use:5ug VSV-G Mouse IgG1 per ml Lysate,WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with VSV-G-tag fusion protein 3、IP(untransfted 293+anti-VSV-G mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-VSV-G mAb+ Protein G agarose) 6、IP (transfected 293+Protein G) 7、Recombinant protein (E.coli)