AR-β2 (phospho Ser355/S356) Polyclonal Antibody
- Catalog No.：YP0712
- Applications：WB;IHC;IF;ELISA
- Reactivity：Human;Mouse;Rat;Monkey
- Target：
- Adrenergic Receptor β2
- Fields：
- >>Calcium signaling pathway;>>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Neuroactive ligand-receptor interaction;>>Adrenergic signaling in cardiomyocytes;>>Regulation of lipolysis in adipocytes;>>Renin secretion;>>Salivary secretion;>>Chemical carcinogenesis - receptor activation
- Gene Name：
- ADRB2
- Protein Name：
- Beta-2 adrenergic receptor
- Human Gene Id：
- 154
- Human Swiss Prot No：
- P07550
- Mouse Gene Id：
- 11555
- Mouse Swiss Prot No：
- P18762
- Rat Swiss Prot No：
- P10608
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human Adrenergic Receptor B2 around the phosphorylation site of Ser355 and Ser356. AA range:331-380
- Specificity：
- Phospho-AR-β2 (S355/S356) Polyclonal Antibody detects endogenous levels of AR-β2 protein only when phosphorylated at S355/S356.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- ADRB2;ADRB2R;B2AR;Beta-2 adrenergic receptor;Beta-2 adrenoreceptor;Beta-2 adrenoceptor
- Observed Band(KD)：
- 47kD
- Background：
- This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008],
- Function：
- disease:Polymorphic forms of ADRB2 could impart some form of nocturnal asthma.,function:Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.,PTM:Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.,PTM:Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the recep
- Subcellular Location：
- Cell membrane ; Multi-pass membrane protein . Early endosome . Golgi apparatus . Colocalizes with VHL at the cell membrane (PubMed:19584355). Activated receptors are internalized into endosomes prior to their degradation in lysosomes (PubMed:20559325). Activated receptors are also detected within the Golgi apparatus (PubMed:27481942). .
- Expression：
- Blood,Brain,Fetal brain,Heart,Leukocyte,Prostate,Thyroid,
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- Antibody-FAQs
- Products Images
- Immunohistochemistry analysis of paraffin-embedded human brain, using Adrenergic Receptor B2 (Phospho-Ser355+Ser356) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from HUVEC cells treated with serum 20% 15' and COS7 cells treated with serum 20% 15', using Adrenergic Receptor B2 (Phospho-Ser355+Ser356) Antibody. The lane on the right is blocked with the phospho peptide.
