AF-4 Polyclonal Antibody

    • Catalog No.:YT0140
    • Applications:IHC-p,IF(paraffin section),WB,ELISA
    • Reactivity:Human,Mouse
      • Gene Name:
      • AFF1
      • Protein Name:
      • AF4/FMR2 family member 1
      • Human Gene Id:
      • 4299
      • Human Swiss Prot No:
      • P51825
      • Mouse Swiss Prot No:
      • O88573
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human AF4. AA range:1-50
      • Specificity:
      • AF-4 Polyclonal Antibody detects endogenous levels of AF-4 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • AFF1; AF4; FEL; MLLT2; PBM1; AF4/FMR2 family member 1; ALL1-fused gene from chromosome 4 protein; Protein AF-4; Protein FEL; Proto-oncogene AF4
      • MolecularWeight(Da):
      • 131422
      • Observed Band(KD):
      • 130
      • Background:
      • AF4/FMR2 family member 1(AFF1) Homo sapiens This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in mu
      • Function:
      • disease:A chromosomal aberration involving AFF1 is associated with acute leukemias. Translocation t(4;11)(q21;q23) with MLL/HRX. The result is a rogue activator protein.,similarity:Belongs to the AF4 family.,
      • Subcellular Location:
      • transcription elongation factor complex,
      • Expression:
      • Epithelium,PCR rescued clones,Placenta,
      • Products Images
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AF4 Antibody. The picture on the right is blocked with the synthesized peptide.