C/EBP α Polyclonal Antibody

    • Catalog No.:YT0552
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Mouse,Rat,Monkey
      • Gene Name:
      • CEBPA
      • Protein Name:
      • CCAAT/enhancer-binding protein alpha
      • Human Gene Id:
      • 1050
      • Human Swiss Prot No:
      • P49715
      • Mouse Swiss Prot No:
      • P53566
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human C/EBP-alpha. AA range:192-241
      • Specificity:
      • C/EBP α Polyclonal Antibody detects endogenous levels of C/EBP α protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • CEBPA; CCAAT/enhancer-binding protein alpha; C/EBP alpha
      • MolecularWeight(Da):
      • 37575
      • Observed Band(KD):
      • 37
      • Background:
      • CCAAT/enhancer binding protein alpha(CEBPA) Homo sapiens This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013],
      • Function:
      • function:C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. C/EBP subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with UBN1. Interacts with HBV protein X.,
      • Subcellular Location:
      • nucleus,nucleolus,RNA polymerase II transcription factor complex,
      • Expression:
      • Liver,Pancreas,Umbilical cord,White Matter pool- 5 brain tissues- f
      • Products Images
      • Western Blot analysis of various cells using C/EBP α Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Western blot analysis of lysates from COS7 cells, treated with EGF 200ng/ml 30', using C/EBP-alpha Antibody. The lane on the right is blocked with the synthesized peptide.