Ikaros(C-term) mouse mAb

    • 货号:YM1278
    • 应用:WB;IP
    • 种属:Human
      • 靶点:
      • Ikaros
      • 基因名称:
      • ikzf1
      • Human Swiss Prot No:
      • Q13422
      • Mouse Swiss Prot No:
      • Q03267
      • 免疫原:
      • Purified recombinant human Ikaros(C-terminus) protein fragments expressed in E.coli.
      • 特异性:
      • This antibody detects endogenous levels of Ikaros(C-terminus) and does not cross-react with related proteins.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Monoclonal, Mouse
      • 稀释:
      • wb 1:1000
      • 纯化工艺:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • CLL associated antigen KW 6;DNA-binding protein Ikaros;hIk 1;hIk-1;Hs.54452;IK1;Ikaros (zinc finger protein);IKAROS;IKAROS family zinc finger 1 (Ikaros);Ikaros family zinc finger protein 1;Ikzf1;IKZF1_HUMAN;LYF1;Lymphoid transcription factor LyF-1;PRO0758;Zinc finger protein subfamily 1A 1 (Ikaros);zinc finger protein subfamily 1A 1;Zinc finger protein, subfamily 1A, member 1;ZNFN1A1.
      • 实测条带:
      • 58kD
      • 背景:
      • This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding
      • 功能:
      • function:Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Functions in the specification and the maturation of the T-lymphocyte. Also interacts with a critical control element in the TDT (terminal deoxynucleotidyltransferase) promoter as well as with the promoters for other genes expressed during early stages of B- and T-cell development.,similarity:Belongs to the Ikaros C2H2-type zinc-finger protein family.,similarity:Contains 6 C2H2-type zinc fingers.,subunit:Interacts with IKZF4 AND IKZF5.,tissue specificity:Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.,
      • 细胞定位:
      • Nucleus . In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events. .; [Isoform Ik2]: Nucleus. In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events (By similarity). .; [Isoform Ik6]: Cytoplasm .
      • 组织表达:
      • Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.

      DDB1 regulates the activation-induced apoptosis of T cells via downregulating the expression of histone methyltransferase SETD7 MEDICAL ONCOLOGY Mao Yong
      货号:YM1278

      • 产品图片
      • Western blot detection of Ikaros(C-terminus) in Ramos and Jurkat cell lysates using Ikaros(C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 58KDa.Observed band size: 58KDa.
      • Immunoprecipitation analysis of Hela cell lysate using Ikaros (C-terminus) mouse mAb.