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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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Rab2 mouse mAb
- 货号:YM1280
- 应用:WB;ICC
- 种属:Human
- 简介:
- >>AMPK signaling pathway
- 免疫原:
- Purified recombinant human Rab2 protein fragments expressed in E.coli.
- 特异性:
- This antibody detects endogenous levels of Rab2 and does not cross-react with related proteins.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 纯化工艺:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- 9330148M11Rik;C80220;GTP binding protein;LHX;OTTMUSP00000004796;RAB2;RAB2 member RAS oncogene family;RAB2A;RAB2A, member RAS oncogene family;RAB2A_HUMAN;Ras related protein Rab 2A;RAS-associated protein RAB2;Ras-related protein Rab-2A;RP23-183L6.1;Small GTP binding protein RAB2A.
- 背景:
- The protein encoded by this gene belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],
- 功能:
- function:Required for protein transport from the endoplasmic reticulum to the Golgi complex.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Interacts with PRKCI.,
- 细胞定位:
- Endoplasmic reticulum-Golgi intermediate compartment membrane ; Lipid-anchor . Melanosome . Endoplasmic reticulum membrane ; Lipid-anchor . Golgi apparatus membrane ; Lipid-anchor . Cytoplasmic vesicle, secretory vesicle, acrosome . Localized in the Golgi apparatus in the round spermatids and in the acrosome in the elongating spermatid (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). .
- Western blot detection of Rab2 in Hela,Ramos and K562 cell lysates and using Rab2 mouse mAb (1:800 diluted).Predicted band size: 24KDa.Observed band size: 24KDa.
- Immunocytochemistry of HeLa cells using anti-Rab2 mouse mAb diluted 1:200