SAPAP1 Polyclonal Antibody

    • 货号:YT4212
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • SAPAP1
      • 简介:
      • >>Glutamatergic synapse
      • 基因名称:
      • DLGAP1
      • 蛋白名称:
      • Disks large-associated protein 1
      • Human Gene Id:
      • 9229
      • Human Swiss Prot No:
      • O14490
      • Mouse Swiss Prot No:
      • Q9D415
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human DLGP1. AA range:791-840
      • 特异性:
      • SAPAP1 Polyclonal Antibody detects endogenous levels of SAPAP1 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • DLGAP1;DAP1;GKAP;Disks large-associated protein 1;DAP-1;Guanylate kinase-associated protein;hGKAP;PSD-95/SAP90-binding protein 1;SAP90/PSD-95-associated protein 1;SAPAP1
      • 实测条带:
      • 108kD
      • 背景:
      • function:Part of the postsynaptic scaffold in neuronal cells.,similarity:Belongs to the SAPAP family.,subcellular location:Found in postsynaptic density of neuronal cells.,subunit:Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1.,tissue specificity:Expressed in brain.,
      • 功能:
      • function:Part of the postsynaptic scaffold in neuronal cells.,similarity:Belongs to the SAPAP family.,subcellular location:Found in postsynaptic density of neuronal cells.,subunit:Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1.,tissue specificity:Expressed in brain.,
      • 细胞定位:
      • Cell membrane ; Peripheral membrane protein . Cell junction, synapse, postsynaptic density . Cell junction, synapse .
      • 组织表达:
      • Expressed in brain.
      • 产品图片
      • Western Blot analysis of various cells using SAPAP1 Polyclonal Antibody
      • Immunofluorescence analysis of HeLa cells, using DLGP1 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HepG2 cells, using DLGP1 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).