CD19 Polyclonal Antibody

    • 货号:YT5580
    • 应用:WB;FCM;IHC;IF;ELISA
    • 种属:Human;Mouse
      • 靶点:
      • CD19
      • 简介:
      • >>PI3K-Akt signaling pathway;>>Hematopoietic cell lineage;>>B cell receptor signaling pathway;>>Epstein-Barr virus infection;>>Primary immunodeficiency
      • 基因名称:
      • CD19
      • 蛋白名称:
      • B-lymphocyte antigen CD19
      • Human Gene Id:
      • 930
      • Human Swiss Prot No:
      • P15391
      • Mouse Swiss Prot No:
      • P25918
      • 免疫原:
      • Synthesized peptide derived from B-lymphocyte antigen CD19 at AA range: 191-240
      • 特异性:
      • CD19 Polyclonal Antibody detects endogenous levels of CD19 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500-2000;Flow Cyt 1:50-200;IHC 1:100-500;IF(paraffin section);ELISA 1:5000-20000
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • CD19;B-lymphocyte antigen CD19;B-lymphocyte surface antigen B4;Differentiation antigen CD19;T-cell surface antigen Leu-12;CD19
      • 实测条带:
      • 61kD
      • 背景:
      • Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008],
      • 功能:
      • disease:Defects in CD19 are a cause of hypogammaglobulinemia [MIM:107265].,function:Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.,online information:CD19 mutation db,PTM:Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.,similarity:Contains 2 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409.,
      • 细胞定位:
      • Cell membrane ; Single-pass type I membrane protein . Membrane raft ; Single-pass type I membrane protein .
      • 组织表达:
      • Detected on marginal zone and germinal center B cells in lymph nodes (PubMed:2463100). Detected on blood B cells (at protein level) (PubMed:2463100, PubMed:16672701).

      Longitudinal Immune Profiling Highlights CD4+ T Cell Exhaustion Correlated with Liver Fibrosis in Schistosoma japonicum Infection JOURNAL OF IMMUNOLOGY Zhiqiang Qin IF,IHC Mouse livers
      货号:YT5580

      • 产品图片
      • Western Blot analysis of mouse brain cells using CD19 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Western Blot analysis of MOUSE-BRAIN cells using CD19 Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100
      • Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100
      • Western Blot analysis of mouse-kidney mouse-spleen using CD19 Polyclonal Antibody diluted at 1:1500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human liver. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).