CD71 (PT0719) Mouse mAb
- Catalog No.:YM4281
- Applications:WB;ELISA
- Reactivity:Human
- Target:
- TFRC
- Gene Name:
- TFRC
- Protein Name:
- Transferrin receptor protein 1 (TR) (TfR) (TfR1) (Trfr) (T9) (p90) (CD antigen CD71) [Cleaved into: Transferrin receptor protein 1, serum form (sTfR)]
- Human Gene Id:
- 7037
- Human Swiss Prot No:
- P02786
- Mouse Gene Id:
- 22042
- Mouse Swiss Prot No:
- Q62351
- Rat Swiss Prot No:
- Q99376
- Immunogen:
- Synthesized peptide derived from human CD71. AA range: 100-200
- Specificity:
- This antibody detects endogenous levels of CD71 at Human
- Formulation:
- Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
- Source:
- Monoclonal, Mouse IgG1, Kappa
- Dilution:
- WB 1:500-2000,ELISA 1:5000-20000
- Purification:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- Transferrin receptor protein 1 (TR) (TfR) (TfR1) (Trfr) (T9) (p90) (CD antigen CD71) [Cleaved into: Transferrin receptor protein 1, serum form (sTfR)]
- Observed Band(KD):
- 95kD
- Background:
- transferrin receptor(TFRC) Homo sapiens This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015],
- Function:
- Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes . Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake . Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway . When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiqu
- Subcellular Location:
- Cell membrane ; Single-pass type II membrane protein . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .; [Transferrin receptor protein 1, serum form]: Secreted .
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- Antibody-FAQs
- Products Images
- Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Her-2 (PT1844) antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody. Lane 1: Hela Lane 2: Jurkat Lane 3: HEK293 Predicted band size: 84kDa Observed band size: 100kDa