• Target：
• Hic-5

• Gene Name：
• TGFB1I1 ARA55

• Protein Name：
• Hic-5 (Phospho-Tyr60)

• Human Gene Id：
• 7041

• Human Swiss Prot No：
• O43294

• Mouse Gene Id：
• 21804

• Mouse Swiss Prot No：
• Q62219

• Rat Gene Id：
• 84574

• Rat Swiss Prot No：
• Q99PD6

• Immunogen：
• Synthesized peptide derived from human Hic-5 (Phospho-Tyr60)

• Specificity：
• This antibody detects endogenous levels of Hic-5 (Phospho-Tyr60) at Human, Mouse,Rat

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000

• Purification：
• The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• Transforming growth factor beta-1-induced transcript 1 protein (Androgen receptor coactivator 55 kDa protein) (Androgen receptor-associated protein of 55 kDa) (Hydrogen peroxide-inducible clone 5 protein) (Hic-5)

• Observed Band(KD)：
• 50kD

• Background：
• transforming growth factor beta 1 induced transcript 1(TGFB1I1) Homo sapiens This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009],

• Function：
• domain:The LD (leucine and aspartate-rich) motif 3 mediates interaction with GIT1 and functions as a nuclear export signal.,domain:The LIM zinc-binding domains mediate glucocorticoid receptor coactivation and interaction with AR, CRIP2, ILK, LIMS1, NR3C1, PPARG, TCF3, TCF7L2, SLC6A3 and SMAD3. The LIM zinc-binding 2 and LIM zinc-binding 3 domains mediate targeting to focal adhesions and actin stress fibers. The LIM zinc-binding 3 and LIM zinc-binding 4 domains mediate interaction with TRAF4 and MAPK15. The LIM zinc-binding 4 domain mediates interaction with HSPB1, homooligomerization and targeting to the nuclear matrix. The LIM zinc-binding 3 domain mediates interaction with PTPN12.,function:Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane

• Subcellular Location：
• Cell junction, focal adhesion. Nucleus matrix. Cytoplasm, cytoskeleton. Associated with the actin cytoskeleton; colocalizes with stress fibers.

• Expression：
• Expressed in platelets, smooth muscle and prostate stromal cells (at protein level).

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