Phospho MEK1/2 (S217) Cell-Based Colorimetric ELISA Kit

  • Catalog No.：KA1606C
  • Applications：ELISA
  • Reactivity：Human;Mouse;Rat
    • Gene Name：
    • MAP2K1/MAP2K2
    • Storage Stability：
    • 2-8°C/6 months
    • Detection Method：
    • Colorimetric
    • Background：
    • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.,enzyme regulation:Activated by phosphorylation.,function:Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.,PTM:Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.,PTM:Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with MORG1 (By similarity). Interacts with Yersinia yopJ.,
    • Function：
    • M phase of mitotic cell cycle, MAPKKK cascade, activation of MAPK activity, mitotic cell cycle, M phase, nuclear division,cell morphogenesis, regulation of vascular smooth muscle contraction, regulation of transcription, DNA-dependent,regulation of transcription from RNA polymerase II promoter, protein complex assembly, protein amino acid phosphorylation, phosphorus metabolic process, phosphate metabolic process, cell motion, chemotaxis, regulation of muscle contraction, regulation of smooth muscle contraction, response to oxidative stress, microtubule-based process,microtubule-based movement, Golgi organization, cell cycle, mitosis, negative regulation of cell adhesion, intracellular signaling cascade, protein kinase cascade, small GTPase mediated signal transduction, Ras protein signal transduction, ectoderm development, behavior, locomotory behavior, cell proliferation, epidermis deve
    • Subcellular Location：
    • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body . Cytoplasm . Nucleus . Membrane ; Peripheral membrane protein . Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis (PubMed:14737111). Membrane localization is probably regulated by its interaction with KSR1 (PubMed:10409742). .
    • Expression：
    • Widely expressed, with extremely low levels in brain.

    Attributes

    • Code：
    • KA1606C
    • Size
    • 96 / well
    • Price$：
    • 470
    • Delivery：
    • 2024-12-08
    Order

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