Total Ref-1 Cell-Based Colorimetric ELISA Kit

  • Catalog No.：KA3575C
  • Applications：ELISA
  • Reactivity：Human;Mouse;Rat
    • Gene Name：
    • APEX1
    • Human Gene Id：
    • 328
    • Human Swiss Prot No：
    • P27695
    • Mouse Swiss Prot No：
    • P28352
    • Storage Stability：
    • 2-8°C/6 months
    • Other Name：
    • DNA-(apurinic or apyrimidinic site) lyase (EC 3.1.-.-) (EC 4.2.99.18) (APEX nuclease) (APEN) (Apurinic-apyrimidinic endonuclease 1) (AP endonuclease 1) (APE-1) (REF-1) (Redox factor-1) [Cleaved into: DNA-(apurinic or apyrimidinic site) lyase, mitochondrial]
    • Detection Method：
    • Colorimetric
    • Background：
    • catalytic activity:The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.,function:Repairs oxidative DNA damages in vitro. May have a role in protection against cell lethality and suppression of mutations. Removes the blocking groups from the 3'-termini of the DNA strand breaks generated by ionizing radiations and bleomycin.,similarity:Belongs to the DNA repair enzymes AP/exoA family.,subunit:Monomer. Component of the SET complex, which also contains SET, ANP32A, HMGB2 and NME1.,
    • Function：
    • DNA metabolic process, DNA repair, base-excision repair, transcription, transcription, DNA-dependent, transcription from RNA polymerase II promoter, response to DNA damage stimulus, cellular homeostasis, RNA biosynthetic process,cellular response to stress, homeostatic process, cell redox homeostasis, regulation of binding, regulation of DNA binding,
    • Subcellular Location：
    • Nucleus. Nucleus, nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm. Detected in the cytoplasm of B-cells stimulated to switch (By similarity). Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 64-80). S-nitrosylation at Cys-93 and

    Attributes

    • Code：
    • KA3575C
    • Size
    • 96 / well
    • Price$：
    • 330
    • Delivery：
    • 2024-12-08
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