Total KSR Cell-Based Colorimetric ELISA Kit

  • Catalog No.：KA4157C
  • Applications：ELISA
  • Reactivity：Human;Mouse
    • Gene Name：
    • KSR1
    • Human Gene Id：
    • 8844
    • Human Swiss Prot No：
    • Q8IVT5
    • Mouse Swiss Prot No：
    • Q61097
    • Storage Stability：
    • 2-8°C/6 months
    • Other Name：
    • Kinase suppressor of Ras 1
    • Detection Method：
    • Colorimetric
    • Background：
    • caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,function:Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.,PTM:Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,subcellular location:In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.,subunit:Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner (By similarity). The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization.,
    • Function：
    • protein amino acid phosphorylation, phosphorus metabolic process, phosphate metabolic process, intracellular signaling cascade, small GTPase mediated signal transduction, Ras protein signal transduction, phosphorylation,
    • Subcellular Location：
    • Cytoplasm . Membrane ; Peripheral membrane protein . Cell membrane ; Peripheral membrane protein . Cell projection, ruffle membrane . Endoplasmic reticulum membrane . In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery. .

    Attributes

    • Code：
    • KA4157C
    • Size
    • 96 / well
    • Price$：
    • 330
    • Delivery：
    • 2024-12-08
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