Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody
- Catalog No.：YC0102
- Applications：WB;IHC;IF;ELISA
- Reactivity：Human;Rat;Mouse;
- Target：
- Plasma Kallikrein HC
- Fields：
- >>Complement and coagulation cascades
- Gene Name：
- KLKB1
- Protein Name：
- Plasma kallikrein
- Human Gene Id：
- 3818
- Human Swiss Prot No：
- P03952
- Mouse Swiss Prot No：
- P26262
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human KLKB1. AA range:341-390
- Specificity：
- Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody detects endogenous levels of fragment of activated Plasma Kallikrein HC protein resulting from cleavage adjacent to R390.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- KLKB1;KLK3;Plasma kallikrein;Fletcher factor;Kininogenin;Plasma prekallikrein
- Observed Band(KD)：
- 45kD
- Background：
- This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016],
- Function：
- catalytic activity:Cleaves selectively Arg-|-Xaa and Lys-|-Xaa bonds, including Lys-|-Arg and Arg-|-Ser bonds in (human) kininogen to release bradykinin.,disease:Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also called Fletcher factor deficiency. This disorder is a blood coagulation defect.,function:The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.,similarity:Belongs to the peptidase S1 family.,similarity:Belongs to the peptidase S1 family. Plasma kallikrein subfamily.,similarity:Contains 1 peptidase S1 domain.,similarity:Contains 4 apple domains.,subunit:The zymogen is activated by factor XIIa, which cleaves t
- Subcellular Location：
- Secreted.
- Expression：
- Colon,Liver,Plasma,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody diluted at 1:1000
.jpg)
- Western Blot analysis of HeLa cells using Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody diluted at 1:1000
- Western blot analysis of lysates from HeLa cells, using KLKB1 (heavy chain,Cleaved-Arg390) Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
