• Gene Name：
• MAP1LC3B

• Protein Name：
• LC3B protein

• Sequence：
• Amino acid: 1-72, with his-MBP tag.

• Human Gene Id：
• 81631

• Human Swiss Prot No：
• Q9GZQ8

• Mouse Swiss Prot No：
• Q9CQV6

• Formulation：
• Liquid in PBS

• Source：
• E.coli

• Dilution：
• WB 1:500-2000

• Concentration：
• SDS-PAGE >90%

• Storage Stability：
• -20°C/6 month,-80°C for long storage

• Other Name：
• Microtubule-associated proteins 1A/1B light chain 3B (Autophagy-related protein LC3 B) (Autophagy-related ubiquitin-like modifier LC3 B) (MAP1 light chain 3-like protein 2) (MAP1A/MAP1B light chain 3 B) (MAP1A/MAP1B LC3 B) (Microtubule-associated protein 1 light chain 3 beta)

• Background：
• caution:PubMed:12740394 has shown that the protein is cleaved at Lys-122 but PubMed:15355958 has shown that the cleavage site is at Gly-120 as in other mammalian orthologs.,function:Probably involved in formation of autophagosomal vacuoles (autophagosomes).,PTM:The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.,similarity:Belongs to the MAP1 LC3 family.,subcellular location:LC3-II binds to the autophagic membranes.,subunit:3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.,tissue specificity:Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.,

• Function：
• proteolysis, autophagy, macromolecule catabolic process, modification-dependent protein catabolic process, protein catabolic process, modification-dependent macromolecule catabolic process, cellular protein catabolic process, cellular macromolecule catabolic process, proteolysis involved in cellular protein catabolic process,

• Subcellular Location：
• Cytoplasmic vesicle, autophagosome membrane ; Lipid-anchor . Endomembrane system ; Lipid-anchor . Mitochondrion membrane ; Lipid-anchor . Cytoplasm, cytoskeleton . Cytoplasmic vesicle . LC3-II binds to the autophagic membranes. LC3-II localizes with the mitochondrial inner membrane during Parkin-mediated mitophagy (PubMed:28017329). Localizes also to discrete punctae along the ciliary axoneme. .

• Expression：
• Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.

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