• Target：
• CD247

• Fields：
• >>Natural killer cell mediated cytotoxicity;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>Chagas disease;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>PD-L1 expression and PD-1 checkpoint pathway in cancer

• Gene Name：
• CD247

• Protein Name：
• T-cell surface glycoprotein CD3 zeta chain

• Human Gene Id：
• 919

• Human Swiss Prot No：
• P20963

• Mouse Swiss Prot No：
• P24161

• Immunogen：
• Purified recombinant fragment of human CD247 expressed in E. Coli.

• Specificity：
• CD247 Monoclonal Antibody detects endogenous levels of CD247 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CD247;CD3Z;T3Z;TCRZ;T-cell surface glycoprotein CD3 zeta chain;T-cell receptor T3 zeta chain;CD antigen CD247

• Molecular Weight(Da)：
• 19kD

• References：
• 1. J Immunol. 2002 Aug 15;169(4):1705-12.
  2. Arthritis Rheum. 2003 Jul;48(7):1948-55.
  3. Nat Methods. 2005 Aug;2(8):591-8.

• Background：
• The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

• Function：
• disease:Defects in CD247 are a cause of primary T-cell immunodeficiency [MIM:610163]. Affected individuals suffer of recurrent infections. Patients T-cell counts are very low and B-cell counts are normal.,domain:The ITAM domains mediate interaction with SHB.,function:Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.,online information:CD247 mutation db,PTM:Phosphorylated on Tyr residues after T-cell receptor triggering.,similarity:Belongs to the CD3Z/FCER1G family.,similarity:Contains 3 ITAM domains.,subunit:The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2. Interacts w

• Subcellular Location：
• Cell membrane ; Single-pass type I membrane protein.

• Expression：
• CD3Z is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs) (PubMed:11722641).

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