Crk-L Monoclonal Antibody
- Catalog No.:YM0168
- Applications:WB;FCM;ELISA
- Reactivity:Human
- Target:
- Crk-L
- Fields:
- >>MAPK signaling pathway;>>ErbB signaling pathway;>>Rap1 signaling pathway;>>Chemokine signaling pathway;>>Focal adhesion;>>Fc gamma R-mediated phagocytosis;>>Neurotrophin signaling pathway;>>Regulation of actin cytoskeleton;>>Insulin signaling pathway;>>Growth hormone synthesis, secretion and action;>>Bacterial invasion of epithelial cells;>>Shigellosis;>>Yersinia infection;>>Human cytomegalovirus infection;>>Human immunodeficiency virus 1 infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Renal cell carcinoma;>>Chronic myeloid leukemia
- Gene Name:
- CRKL
- Protein Name:
- Crk-like protein
- Human Gene Id:
- 1399
- Human Swiss Prot No:
- P46109
- Mouse Swiss Prot No:
- P47941
- Immunogen:
- Purified recombinant fragment of human Crk-L expressed in E. Coli.
- Specificity:
- Crk-L Monoclonal Antibody detects endogenous levels of Crk-L protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Monoclonal, Mouse
- Dilution:
- WB 1:500 - 1:2000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
- Purification:
- Affinity purification
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- CRKL;Crk-like protein
- Molecular Weight(Da):
- 34kD
- References:
- 1. Mol Cell Biol. 2009 Jun;29(11):3076-87.
2. Cell. 2009 Jul 23;138(2):389-403.
- Background:
- This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009],
- Function:
- function:May mediate the transduction of intracellular signals.,similarity:Contains 1 SH2 domain.,similarity:Contains 2 SH3 domains.,subunit:Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB and IRS4.,
- Subcellular Location:
- endosome,cytosol,cell-cell adherens junction,extracellular exosome,
- Expression:
- Skin,Spleen,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
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- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
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- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
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- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis using Crk-L Monoclonal Antibody against human Crk-L (AA: 100-303) recombinant protein.
- Flow cytometric analysis of NIH/3T3 cells using Crk-L Monoclonal Antibody (blue) and negative control (red).