• Target：
• Crystallin-αB

• Fields：
• >>Protein processing in endoplasmic reticulum;>>Longevity regulating pathway - multiple species

• Gene Name：
• CRYAB

• Protein Name：
• Alpha-crystallin B chain

• Human Gene Id：
• 1410

• Human Swiss Prot No：
• P02511

• Mouse Swiss Prot No：
• P23927

• Immunogen：
• Purified recombinant fragment of Crystallin-αB (aa1-175) expressed in E. Coli.

• Specificity：
• Crystallin-αB Monoclonal Antibody detects endogenous levels of Crystallin-αB protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. ELISA: 1:10000.. IF 1:50-200

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CRYAB;CRYA2;Alpha-crystallin B chain;Alpha(B)-crystallin;Heat shock protein beta-5;HspB5;Renal carcinoma antigen NY-REN-27;Rosenthal fiber component

• Molecular Weight(Da)：
• 20kD

• References：
• 1. Cell. 2007 Aug 10;130(3):427-39.
  2. Biochemistry. 2006 Nov 21;45(46):13847-54.
  3. J Mol Biol. 2007 Sep 14;372(2):470-84.

• Background：
• Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distin

• Function：
• disease:Crystallins do not turn over as the lens ages, providing ample opportunity for post-translational modifications or oxidations. These modifications may change crystallin solubility properties and favor senile cataract.,disease:Defects in CRYAB are the cause of alpha-B crystallinopathy [MIM:608810]. Alpha-B crystallinopathy is a an autosomal dominant form of desmin-related myopathy (DRM) that results in weakness of the proximal and distal limb muscle (including neck, velopharynx, and trunk muscles), signs of cardiomyopathy and cataract. Patients with progressive myopathy characterized by myofibrillar degeneration that commences at the Z-disk, have been described. Mutations truncate the essential C-terminal domain of the protein required for the chaperone function.,disease:Seen as Rosenthal fiber protein in the brain tissue of patients with Alexander disease.,function:May contribute

• Subcellular Location：
• Cytoplasm . Nucleus . Secreted . Lysosome . Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (PubMed:19464326). Localizes at the Z-bands and the intercalated disk in cardiomyocytes (PubMed:28493373). Can be secreted; the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion (PubMed:32272059). .

• Expression：
• Lens as well as other tissues (PubMed:838078, PubMed:2387586). Expressed in myocardial tissue (PubMed:28493373).

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