• Target：
• Emt

• Fields：
• >>Chemokine signaling pathway;>>T cell receptor signaling pathway;>>Leukocyte transendothelial migration

• Gene Name：
• ITK

• Protein Name：
• Tyrosine-protein kinase ITK/TSK

• Human Gene Id：
• 3702

• Human Swiss Prot No：
• Q08881

• Mouse Swiss Prot No：
• Q03526

• Immunogen：
• Purified recombinant fragment of human Emt expressed in E. Coli.

• Specificity：
• Emt Monoclonal Antibody detects endogenous levels of Emt protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ITK;EMT;LYK;Tyrosine-protein kinase ITK/TSK;Interleukin-2-inducible T-cell kinase;IL-2-inducible T-cell kinase;Kinase EMT;T-cell-specific kinase;Tyrosine-protein kinase Lyk

• Molecular Weight(Da)：
• 72kD

• References：
• 1. J Clin Invest. 2009 May;119(5):1350-8.
  2. Mol Cells. 2009 Aug 31;28(2):125-30.

• Background：
• This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 1 zinc ion per subunit.,function:Plays a role in T-cell proliferation and differentiation.,induction:By interleukin-2.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.,similarity:Contains 1 Btk-type zinc finger.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.,tissue specificity:T-cell lines and natural killer cell lines.,

• Subcellular Location：
• Cytoplasm . Nucleus . Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.

• Expression：
• T-cell lines and natural killer cell lines.

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