• Target：
• EphA4

• Fields：
• >>Axon guidance

• Gene Name：
• EPHA4

• Protein Name：
• Ephrin type-A receptor 4

• Human Gene Id：
• 2043

• Human Swiss Prot No：
• P54764

• Mouse Swiss Prot No：
• Q03137

• Immunogen：
• Purified recombinant fragment of EphA4 (aa777-986) expressed in E. Coli.

• Specificity：
• EphA4 Monoclonal Antibody detects endogenous levels of EphA4 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• EPHA4;HEK8;SEK;TYRO1;Ephrin type-A receptor 4;EPH-like kinase 8;EK8;hEK8;Tyrosine-protein kinase TYRO1;Tyrosine-protein kinase receptor SEK

• Molecular Weight(Da)：
• 110kD

• References：
• 1. Mol Cell Neurosci. 2000 Oct;16(4):365-75.
  2. Nat Rev Neurosci. 2001 Mar;2(3):155-64.
  3. Eur J Neurosci. 2002 Sep;16(6):1168-72.

• Background：
• This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015],

• Function：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,domain:The protein kinase domain mediates interaction with NGEF/ephexin-1.,function:Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in a signal transduction process involved in hindbrain pattern formation.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 fibronectin type-III domains.,subunit:Interacts with the src family kinase, p59-Fyn, through the major phosphorylation site at position Tyr-602. Interacts with NGEF/ephexin-1.,tissue specificity:Ubiquitous.

• Subcellular Location：
• Cell membrane ; Single-pass type I membrane protein . Cell projection, axon . Cell projection, dendrite . Cell junction, synapse, postsynaptic density membrane . Early endosome . Cell junction, adherens junction . Clustered upon activation and targeted to early endosome. .

• Expression：
• Ubiquitous.

Identification of WISP1 as a novel oncogene in glioblastoma. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2017 Oct;51(4):1261-1270 WB Human 1:1000 U251 cell, U373-MG cell

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