• Target：
• Flk-1/VEGFR2

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>Calcium signaling pathway;>>PI3K-Akt signaling pathway;>>VEGF signaling pathway;>>Focal adhesion;>>Proteoglycans in cancer;>>Fluid shear stress and atherosclerosis

• Gene Name：
• KDR

• Protein Name：
• Vascular endothelial growth factor receptor 2

• Human Gene Id：
• 3791

• Human Swiss Prot No：
• P35968

• Mouse Swiss Prot No：
• P35918

• Immunogen：
• Purified recombinant extracellular fragment of human Flk-1 (aa20-764) fused with hIgGFc tag expressed in HEK293 cells.

• Specificity：
• Flk-1 Monoclonal Antibody detects endogenous levels of Flk-1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• KDR;FLK1;VEGFR2;Vascular endothelial growth factor receptor 2;VEGFR-2;Fetal liver kinase 1;FLK-1;Kinase insert domain receptor;KDR;Protein-tyrosine kinase receptor flk-1;CD antigen CD309

• Molecular Weight(Da)：
• 152kD

• References：
• 1. Blood. 2004 Aug 1;104(3):788-94.
  2. FEBS Lett. 2002 Feb 13;512(1-3):107-10.
  3. EMBO J. 2001 Jun 1;20(11):2768-78.

• Background：
• Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009],

• Function：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 7 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Interacts with MYOF (By similarity). Interacts with SHB; upon VEGF activation. Interacts with HIV-1 Tat.,

• Subcellular Location：
• Cell junction . Endoplasmic reticulum . Cell membrane . Localized with RAP1A at cell-cell junctions (By similarity). Colocalizes with ERN1 and XBP1 in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (PubMed:23529610). .; [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome. Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus.; [Isoform 2]: Secreted .; [Isoform 3]: Secreted.

• Expression：
• Detected in cornea (at protein level). Widely expressed.

DOT1L promotes angiogenesis through cooperative regulation of VEGFR2 with ETS-1. Oncotarget Oncotarget. 2016 Oct 25; 7(43): 69674–69687 ChIP Human HUVECs

Exposure to Intermittent Environmental Hypoxia Promotes Vascular Remodeling through Angiogenesis in the Liver of Largemouth Bass (Micropterus salmoides) ENVIRONMENTAL SCIENCE & TECHNOLOGY Song Yang IF Fish liver

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