• Target：
• HGK

• Fields：
• >>MAPK signaling pathway

• Gene Name：
• MAP4K4

• Protein Name：
• Mitogen-activated protein kinase kinase kinase kinase 4

• Human Gene Id：
• 9448

• Human Swiss Prot No：
• O95819

• Mouse Swiss Prot No：
• P97820

• Immunogen：
• Purified recombinant fragment of HGK (aa400-500) expressed in E. Coli.

• Specificity：
• HGK Monoclonal Antibody detects endogenous levels of HGK protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MAP4K4;HGK;KIAA0687;NIK;Mitogen-activated protein kinase kinase kinase kinase 4;HPK/GCK-like kinase HGK;MAPK/ERK kinase kinase kinase 4;MEK kinase kinase 4;MEKKK 4;Nck-interacting kinase

• Molecular Weight(Da)：
• 142kD

• References：
• 1. Mol Cell Biol. 2000 Mar;20(5):1537-45.
  2. Curr Biol. 2002 Apr 16;12(8):622-31.
  3. J Biol Chem. 2007 Mar 16;282(11):7783-9.

• Background：
• mitogen-activated protein kinase kinase kinase kinase 4(MAP4K4) Homo sapiens The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 CNH domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with the SH3 domain of the adapter proteins Nck (By similarity). Binds, via its CNH regulatory domain, to the N-terminal region of SPG3A.,tissue specificity:Appears to be ubiquitous, expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain, isoform 4 is predominant in the liver, skelet

• Subcellular Location：
• Cytoplasm .

• Expression：
• Widely expressed. Isoform 5 is abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.

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