JMJD1A Monoclonal Antibody
- Catalog No.：YM0388
- Applications：WB;IF;ELISA
- Reactivity：Human
- Target：
- JMJD1A
- Fields：
- >>Thermogenesis
- Gene Name：
- KDM3A
- Protein Name：
- Lysine-specific demethylase 3A
- Human Gene Id：
- 55818
- Human Swiss Prot No：
- Q9Y4C1
- Mouse Swiss Prot No：
- Q6PCM1
- Immunogen：
- Purified recombinant fragment of human JMJD1A expressed in E. Coli.
- Specificity：
- JMJD1A Monoclonal Antibody detects endogenous levels of JMJD1A protein.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Monoclonal, Mouse
- Dilution：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- Purification：
- Affinity purification
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- KDM3A;JHDM2A;JMJD1;JMJD1A;KIAA0742;TSGA;Lysine-specific demethylase 3A;JmjC domain-containing histone demethylation protein 2A;Jumonji domain-containing protein 1A
- Molecular Weight(Da)：
- 147kD
- References：
- 1. DNA Res. 1998 Oct 30;5(5):277-86.
2. Proc Natl Acad Sci U S A.2004 Aug 17;101(33):12130-5.
3. Nature. 2005 Apr 7;434(7034):724-31.
- Background：
- This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009],
- Function：
- cofactor:Binds 1 Fe(2+) ion per subunit.,domain:Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.,domain:The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.,function:Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1
- Subcellular Location：
- Cytoplasm . Nucleus . Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity). .
- Expression：
- Adrenal gland,Brain,Fetal kidney,Salivary gland,Testis,
KDM3A promotes inhibitory cytokines secretion by participating in TLR4 regulation of Foxp3 transcription in lung adenocarcinoma cells. Oncology Letters Oncol Lett. 2017 May;13(5):3529-3537 WB,IF Human 1:1000,1:1000 A549 cell
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- Antibody-FAQs
- Products Images
- Western Blot analysis using JMJD1A Monoclonal Antibody against HeLa (1) and HepG2 (2) cell lysate.
- Immunofluorescence analysis of Hela cells using JMJD1A Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
