• Target：
• JMJD1A

• Fields：
• >>Thermogenesis

• Gene Name：
• KDM3A

• Protein Name：
• Lysine-specific demethylase 3A

• Human Gene Id：
• 55818

• Human Swiss Prot No：
• Q9Y4C1

• Mouse Swiss Prot No：
• Q6PCM1

• Immunogen：
• Purified recombinant fragment of human JMJD1A expressed in E. Coli.

• Specificity：
• JMJD1A Monoclonal Antibody detects endogenous levels of JMJD1A protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• KDM3A;JHDM2A;JMJD1;JMJD1A;KIAA0742;TSGA;Lysine-specific demethylase 3A;JmjC domain-containing histone demethylation protein 2A;Jumonji domain-containing protein 1A

• Molecular Weight(Da)：
• 147kD

• References：
• 1. DNA Res. 1998 Oct 30;5(5):277-86.
  2. Proc Natl Acad Sci U S A.2004 Aug 17;101(33):12130-5.
  3. Nature. 2005 Apr 7;434(7034):724-31.

• Background：
• This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009],

• Function：
• cofactor:Binds 1 Fe(2+) ion per subunit.,domain:Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.,domain:The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.,function:Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1

• Subcellular Location：
• Cytoplasm . Nucleus . Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity). .

• Expression：
• Adrenal gland,Brain,Fetal kidney,Salivary gland,Testis,

KDM3A promotes inhibitory cytokines secretion by participating in TLR4 regulation of Foxp3 transcription in lung adenocarcinoma cells. Oncology Letters Oncol Lett. 2017 May;13(5):3529-3537 WB,IF Human 1:1000,1:1000 A549 cell

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