• Target：
• Lsk

• Fields：
• >>Neurotrophin signaling pathway

• Gene Name：
• MATK

• Protein Name：
• Megakaryocyte-associated tyrosine-protein kinase

• Human Gene Id：
• 4145

• Human Swiss Prot No：
• P42679

• Mouse Swiss Prot No：
• P41242

• Immunogen：
• Purified recombinant fragment of human Lsk expressed in E. Coli.

• Specificity：
• Lsk Monoclonal Antibody detects endogenous levels of Lsk protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MATK;CTK;HYL;Megakaryocyte-associated tyrosine-protein kinase;CSK homologous kinase;CHK;Hematopoietic consensus tyrosine-lacking kinase;Protein kinase HYL;Tyrosine-protein kinase CTK

• Molecular Weight(Da)：
• 56kD

• References：
• 1. Int J Oncol. 2002 Jul;21(1):197-205.
  2. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903.
  3. Nat Genet. 2004 Jan;36(1):40-5.

• Background：
• The protein encoded by this gene has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site. This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,tissue specificity:Expressed in various myeloid cell lines, detected in brain and lung.,

• Subcellular Location：
• Cytoplasm . Membrane . In platelets, 90% of MATK localizes to the membrane fraction, and translocates to the cytoskeleton upon thrombin stimulation.

• Expression：
• Expressed in various myeloid cell lines, detected in brain and lung.

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