• Target：
• Prohibitin

• Gene Name：
• PHB

• Protein Name：
• Prohibitin

• Human Gene Id：
• 5245

• Human Swiss Prot No：
• P35232

• Mouse Gene Id：
• 18673

• Mouse Swiss Prot No：
• P67778

• Rat Gene Id：
• 25344

• Rat Swiss Prot No：
• P67779

• Immunogen：
• Purified recombinant fragment of human Prohibitin expressed in E. Coli.

• Specificity：
• Prohibitin Monoclonal Antibody detects endogenous levels of Prohibitin protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PHB;Prohibitin

• Molecular Weight(Da)：
• 30kD

• References：
• 1. Biochem Biophys Res Commun. 2009 Dec 18;390(3):1023-8.
  2. J Cell Biochem. 2009 Nov 1;108(4):926-34.

• Background：
• prohibitin(PHB) Homo sapiens This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],

• Function：
• developmental stage:Levels of expression in fibroblasts decrease heterogeneously during cellular aging.,disease:Mutated in sporadic breast cancer.,function:Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.,induction:Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. Also induced following inhibition of mitochondrial protein synthesis by thiamphenicol.,similarity:Belongs to the prohibitin family.,subunit:Interacts with PHB2.,tissue specificity:Widely expressed in different tissues.,

• Subcellular Location：
• Mitochondrion inner membrane . Nucleus . Cytoplasm . Cell membrane .

• Expression：
• Widely expressed in different tissues.

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