• Target：
• SIRT1

• Fields：
• >>Nicotinate and nicotinamide metabolism;>>Metabolic pathways;>>FoxO signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Glucagon signaling pathway;>>Alcoholic liver disease;>>Amphetamine addiction;>>MicroRNAs in cancer

• Gene Name：
• SIRT1

• Protein Name：
• NAD-dependent deacetylase sirtuin-1

• Human Gene Id：
• 23411

• Human Swiss Prot No：
• Q96EB6

• Mouse Swiss Prot No：
• Q923E4

• Immunogen：
• Purified recombinant fragment of human SIRT1 expressed in E. Coli.

• Specificity：
• SIRT1 Monoclonal Antibody detects endogenous levels of SIRT1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• SIRT1;SIR2L1;NAD-dependent protein deacetylase sirtuin-1;hSIRT1;Regulatory protein SIR2 homolog 1;SIR2-like protein 1;hSIR2

• Molecular Weight(Da)：
• 82kD

• References：
• 1. Clin Cancer Res. 2009 Jul 1;15(13):4453-9.
  2. Cell. 2009 Jul 23;138(2):389-403.
  3. J Biol Chem. 2009 Oct 16;284(42):28762-74.

• Background：
• This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008],

• Function：
• catalytic activity:NAD(+) + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.,cofactor:Binds 1 zinc ion per subunit.,enzyme regulation:Inhibited by nicotinamide. Activated by resveratrol (3,5,4'-trihydroxy-trans-stilbene), butein (3,4,2',4'-tetrahydroxychalcone), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene), Isoliquiritigenin (4,2',4'-trihydroxychalcone), fisetin (3,7,3',4'-tetrahydroxyflavone) and quercetin (3,5,7,3',4'-pentahydroxyflavone). RPS19BP1/AROS acts as a positive regulator of deacetylation activity.,function:NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1

• Subcellular Location：
• Nucleus, PML body . Cytoplasm . Nucleus . Recruited to the nuclear bodies via its interaction with PML (PubMed:12006491). Colocalized with APEX1 in the nucleus (PubMed:19934257). May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane (PubMed:15469825). Shuttles between nucleus and cytoplasm (By similarity). Colocalizes in the nucleus with XBP1 isoform 2 (PubMed:20955178). .; [SirtT1 75 kDa fragment]: Cytoplasm . Mitochondrion .

• Expression：
• Widely expressed.

Alpha-lipoic acid upregulates SIRT1-dependent PGC-1α expression and protects mouse brain against focal ischemia. NEUROSCIENCE Neuroscience. 2014 Dec;281:251 WB,IHC Mouse 1:200, 1:200 brain,control cortex

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs