• Target：
• Synaptotagmin 1/2

• Fields：
• >>Synaptic vesicle cycle

• Gene Name：
• SYT1

• Protein Name：
• Synaptotagmin-1

• Human Gene Id：
• 6857

• Human Swiss Prot No：
• P21579

• Mouse Swiss Prot No：
• P46096

• Immunogen：
• Purified recombinant fragment of Synaptotagmin 1 expressed in E. Coli.

• Specificity：
• Synaptotagmin 1 Monoclonal Antibody detects endogenous levels of Synaptotagmin 1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• SYT1;SVP65;SYT;Synaptotagmin-1;Synaptotagmin I;SytI;p65

• References：
• 1. Cell. 2005 Sep 23;122(6):957-68.
  2. FASEB J. 2008 Jun;22(6):2045-52.

• Background：
• The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010],

• Function：
• cofactor:Binds 3 calcium ions per subunit. The ions are bound to the C2 domains.,domain:The first C2 domain mediates Ca(2+)-dependent phospholipid binding.,domain:The second C2 domain mediates interaction with SV2A and STN2.,function:May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.,similarity:Belongs to the synaptotagmin family.,similarity:Contains 2 C2 domains.,subcellular location:Synaptic vesicles and chromaffin granules.,subunit:H

• Subcellular Location：
• Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane ; Single-pass membrane protein . Cytoplasm .

• Expression：
• Expressed in melanocytes (PubMed:23999003).

BDNF-Related Imbalance of Copine 6 and Synaptic Plasticity Markers Couples With Depression-Like Behavior and Immune Activation in CUMS Rats. Frontiers in Neuroscience Front Neurosci-Switz. 2018 Oct;0:731 WB Rat 1:1000 hippocampus,prefrontal cortex (PFC)

Protective effect of quercetin against the metabolic dysfunction of glucose and lipids and its associated learning and memory impairments in NAFLD rats. Lipids in Health and Disease Lipids Health Dis. 2021 Dec;20(1):1-15 WB Rat 1:1000 Hippocampus

Resveratrol improved the spatial learning and memory in subclinical hypothyroidism rat induced by hemi-thyroid electrocauterization. ENDOCRINE JOURNAL 2015 Jul 29 WB Rat 1:1000 hippocampi

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs