PP2A-Cα Monoclonal Antibody

    • Catalog No.：YM1079
    • Applications：WB;IF
    • Reactivity：Human;Mouse;Bovine;Chicken;Dog;Pig;Rabbit;Zebrafish
      • Target：
      • PP2AA
      • Fields：
      • >>mRNA surveillance pathway;>>Sphingolipid signaling pathway;>>Oocyte meiosis;>>Autophagy - other;>>Autophagy - animal;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Adrenergic signaling in cardiomyocytes;>>TGF-beta signaling pathway;>>Hippo signaling pathway;>>Tight junction;>>Dopaminergic synapse;>>Long-term depression;>>Chagas disease;>>Hepatitis C;>>Human papillomavirus infection
      • Gene Name：
      • PPP2CA
      • Protein Name：
      • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
      • Human Gene Id：
      • 5515
      • Human Swiss Prot No：
      • P67775
      • Mouse Swiss Prot No：
      • P63330
      • Immunogen：
      • Purified recombinant human PP2A-Cα (N-terminus) protein fragments expressed in E.coli.
      • Specificity：
      • PP2A-Cα Monoclonal Antibody detects endogenous levels of PP2A-Cα protein.
      • Formulation：
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source：
      • Monoclonal, Mouse
      • Dilution：
      • WB 1:1000 - 1:2000. IF 1:100 - 1:500. Not yet tested in other applications.
      • Purification：
      • Affinity purification
      • Concentration：
      • 1 mg/ml
      • Storage Stability：
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name：
      • PPP2CA;Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform;PP2A-alpha;Replication protein C;RP-C
      • Molecular Weight(Da)：
      • 36kD
      • Background：
      • This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008],
      • Function：
      • catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,cofactor:Binds 1 iron ion per subunit.,cofactor:Binds 1 manganese ion per subunit.,function:PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.,PTM:Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation
      • Subcellular Location：
      • Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres (PubMed:16541025). During mitosis, also found at spindle poles (PubMed:16541025). Centromeric localization requires the presence of SGO2 (By similarity). .
      • Expression：
      • Fibroblast,Liver,Lung,Placenta,Testis,Uterus,
      • Products Images
      • Western Blot analysis using PP2A-Cα Monoclonal Antibody against MCF7 cell lysate.
      • Immunofluorescence analysis of HeLa cells using PP2A-Cα Monoclonal Antibody.

      Attributes

      • Code：
      • YM1079
      • Size
      • 50μL / 100μL / 200μL
      • Price$：
      • 210 / 350 / 600
      • Delivery：
      • 2024-05-09
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