• Target：
• PP2AA

• Fields：
• >>mRNA surveillance pathway;>>Sphingolipid signaling pathway;>>Oocyte meiosis;>>Autophagy - other;>>Autophagy - animal;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Adrenergic signaling in cardiomyocytes;>>TGF-beta signaling pathway;>>Hippo signaling pathway;>>Tight junction;>>Dopaminergic synapse;>>Long-term depression;>>Chagas disease;>>Hepatitis C;>>Human papillomavirus infection

• Gene Name：
• PPP2CA

• Protein Name：
• Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

• Human Gene Id：
• 5515

• Human Swiss Prot No：
• P67775

• Mouse Gene Id：
• 19052

• Mouse Swiss Prot No：
• P63330

• Rat Gene Id：
• 24672

• Rat Swiss Prot No：
• P63331

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human PP2A-alpha around the phosphorylation site of Tyr307. AA range:260-309

• Specificity：
• Phospho-PP2A-Cα (Y307) Polyclonal Antibody detects endogenous levels of PP2A-Cα protein only when phosphorylated at Y307.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PPP2CA;Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform;PP2A-alpha;Replication protein C;RP-C

• Observed Band(KD)：
• 35kD

• Background：
• This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,cofactor:Binds 1 iron ion per subunit.,cofactor:Binds 1 manganese ion per subunit.,function:PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.,PTM:Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation

• Subcellular Location：
• Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres (PubMed:16541025). During mitosis, also found at spindle poles (PubMed:16541025). Centromeric localization requires the presence of SGO2 (By similarity). .

• Expression：
• Fibroblast,Liver,Lung,Placenta,Testis,Uterus,

Microcystin-Leucine-Arginine Induces Tau Pathology Through Bα Degradation via Protein Phosphatase 2A Demethylation and Associated Glycogen Synthase Kinase-3β Phosphorylation. TOXICOLOGICAL SCIENCES 2018 Apr 01 IP Human SH-SY5Y cell

Zhang, Yali, et al. "Microcystin-LR induces tau pathology through Bα degradation via PP2A demethylation and associated GSK-3β phosphorylation." Toxicological Sciences(2017).

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