• Target：
• UHRF1

• Gene Name：
• UHRF1

• Protein Name：
• E3 ubiquitin-protein ligase UHRF1

• Human Gene Id：
• 29128

• Human Swiss Prot No：
• Q96T88

• Mouse Swiss Prot No：
• Q8VDF2

• Immunogen：
• Purified recombinant human UHRF1 (N-terminus) protein fragments expressed in E.coli.

• Specificity：
• UHRF1 Monoclonal Antibody detects endogenous levels of UHRF1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:1000 - 1:2000. IF 1:100 - 1:500. Not yet tested in other applications.

• Purification：
• Affinity purification

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• UHRF1;ICBP90;NP95;RNF106;E3 ubiquitin-protein ligase UHRF1;Inverted CCAAT box-binding protein of 90 kDa;Nuclear protein 95;Nuclear zinc finger protein Np95;HuNp95;hNp95;RING finger protein 106;Transcription factor ICBP90;Ubiquit

• Molecular Weight(Da)：
• 90kD

• Background：
• This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014],

• Function：
• developmental stage:Expressed in fetal thymus, liver and kidney.,domain:The RING finger is required for ubiquitin ligase activity.,domain:The YDG domain mediates the interaction with histone H3.,function:Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.,induction:Up-regulated in proliferating cells, and down-regulated in quiescent cells. Down-regulated upon adriamycin-induced DNA damage, in a TP53/p53 and CDKN1A-dependent way. Induced by E2F1 transcription factor.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.,PTM:Ubiquitinated; which leads to proteasomal degrad

• Subcellular Location：
• Nucleus . Localizes to replication foci. Enriched in pericentric heterochromatin. Also localizes to euchromatic regions.

• Expression：
• Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.

Pi, Jing-Ting, et al. "Overexpression of UHRF1 is significantly associated with poor prognosis in laryngeal squamous cell carcinoma." Medical oncology 30.4 (2013): 613.

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