• Target：
• Fatty Acid Synthase

• Fields：
• >>Fatty acid biosynthesis;>>Metabolic pathways;>>Fatty acid metabolism;>>AMPK signaling pathway;>>Insulin signaling pathway;>>Alcoholic liver disease

• Gene Name：
• fasn

• Human Gene Id：
• 2194

• Human Swiss Prot No：
• P49327

• Mouse Swiss Prot No：
• P19096

• Immunogen：
• Purified recombinant human Fatty Acid Synthase protein fragments expressed in E.coli.

• Specificity：
• This antibody detects endogenous levels of Fatty Acid Synthase and does not cross-react with related proteins.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• wb 1:1000 icc 1:400. IF 1:50-200

• Purification：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• [Acyl-carrier-protein] S acetyltransferase;[Acyl-carrier-protein] S malonyltransferase;3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase;3-oxoacyl-[acyl-carrier-protein] reductase;3-oxoacyl-[acyl-carrier-protein] synthase;Enoyl-[acyl-carrier-protein]

• Observed Band(KD)：
• 273kD

• Background：
• The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH.,catalytic activity:(3R)-3-hydroxypalmitoyl-[acyl-carrier-protein] = hexadec-2-enoyl-[acyl-carrier-protein] + H(2)O.,catalytic activity:Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein].,catalytic activity:Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO(2) + 2n NADP(+).,catalytic activity:Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein].,catalytic activity:Acyl-[acyl-carrier-protein] + NADP(+) = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADPH.,catalytic activity:Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein].,catalytic activity:Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate.,functi

• Subcellular Location：
• Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

• Expression：
• Ubiquitous. Prominent expression in brain, lung, liver and mammary gland.

Sirtuin 1 is involved in oleic acid-induced calf hepatocyte steatosis via alterations in lipid metabolism-related proteins. JOURNAL OF ANIMAL SCIENCE J Anim Sci. 2021 Aug;: WB Cow 1:500 Hepatocytes

Low Expression of Sirtuin 1 in the Dairy Cows with Mild Fatty Liver Alters Hepatic Lipid Metabolism. Animals Animals-Basel. 2020 Apr;10(4):560 WB Bovine Liver

PITPNC1 Suppress CD8+ T cell immune function and promote radioresistance in rectal cancer by modulating FASN/CD155 Journal of Translational Medicine Liang Junxian IF Mouse,Human 1:200 MC38 cell-Xenograft HT29 cell,SW480 cell

Perfluorooctane sulfonate causes pyroptosis and lipid metabolism disorders through ROS-mediated NLRP3 inflammasome activation in grass carp hepatocyte AQUATIC TOXICOLOGY Bendong Shi WB Fish L8824 cell

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