• Target：
• Tubulin β

• Fields：
• >>Phagosome;>>Gap junction;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Pathogenic Escherichia coli infection;>>Salmonella infection

• Gene Name：
• TUBB3

• Protein Name：
• Tubulin beta-3 chain

• Human Gene Id：
• 10381

• Human Swiss Prot No：
• Q13509

• Mouse Gene Id：
• 22152

• Mouse Swiss Prot No：
• Q9ERD7

• Rat Gene Id：
• 246118

• Rat Swiss Prot No：
• Q4QRB4

• Immunogen：
• Synthetic Peptide of β-Tubulin

• Specificity：
• The antibody detects endogenous β-tubulin protein.

• Formulation：
• PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:5000 IHC 1:200 IF 1:200

• Purification：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• TUBB3;TUBB4;Tubulin beta-3 chain;Tubulin beta-4 chain;Tubulin beta-III

• Observed Band(KD)：
• 55kD

• Background：
• tubulin beta 3 class III(TUBB3) Homo sapiens This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010],

• Function：
• domain:The highly acidic C-terminal region may bind cations such as calcium.,function:Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.,function:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.,polymorphism:Genetic variations in MC1R are associated with variation in skin/hair/eye pigmentation type 2 (SHEP2) [MIM:266300]. Hair, eye and skin pigmentation are among the most visible examples of human phenotypic variation, with a broad normal range that is subject to substantial geographic stratification. In the case of skin, individuals tend to have lighter pigmentation with increasing distance from the equator. By contrast, the majority of variation in human eye and hair col

• Subcellular Location：
• Cytoplasm, cytoskeleton . Cell projection, growth cone . Cell projection, lamellipodium . Cell projection, filopodium .

• Expression：
• Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.

Exosomal thioredoxin-1 from hypoxic human umbilical cord mesenchymal stem cells inhibits ferroptosis in doxorubicin-induced cardiotoxicity via mTORC1 signaling

Hsa_circ_0001550 impairs decidualization by regulating the proliferation and apoptosis of endometrial stromal cells

EMT status of circulating breast cancer cells and impact of fluidic shear stress

Preliminary verification of the anti-hypoxia mechanism of Gentiana straminea maxim based on UPLC-triple TOF MS/MS and network pharmacology

Large-scale chromatin reorganization reactivates placenta-specific genes that drive cellular aging WB Human /hMPCs

HOXC6 Regulates the Epithelial-Mesenchymal Transition through the TGF-β/Smad Signaling Pathway and Predicts a Poor Prognosis in Glioblastoma WB Human glioblastoma/U251 cell, U87 cell, A172 cell, T98G cell, H4 cell, SHG44 cell

LncRNA TUG1 Promotes Apoptosis, Invasion, and Angiogenesis of Retinal Endothelial Cells in Retinopathy of Prematurity via MiR-145-5p. Frontiers in Medicine2022 Apr 4;9:803214. Mouse 1:1000 retinal tissue

KLF2 up-regulates IRF4/HDAC7 to protect neonatal rats from hypoxic-ischemic brain damage Cell Death Discov. 2022 Jan;8(1):1-10. IF Rat 1:200 cerebral cortex

The Long-Noncoding RNA TUG1 Regulates Oxygen-Induced Retinal Neovascularization in Mice via MiR-299 Invest Ophth Vis Sci. 2022 Jan;63(1):37-37. WB Mouse 1:1000 retina

SIRT3 consolidates heterochromatin and counteracts senescence. NUCLEIC ACIDS RESEARCH Nucleic Acids Res. 2021 May;49(8):4203-4219 WB Human hMSCs

Zinc transporter SLC39A13/ZIP13 facilitates the metastasis of human ovarian cancer cells via activating Src/FAK signaling pathway. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH J Exp Clin Canc Res. 2021 Dec;40(1):1-15 WB Human SKOV-3 cell，HO-8910 PM cell，HEK 293T cell

CD38 affects the biological behavior and energy metabolism of nasopharyngeal carcinoma cells. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2019 Feb;54(2):585-599 WB Human 1:5000 5-8F cell

Protective Mechanism of Adipose-Derived Stem Cells in Remodelling of the Skin Stem Cell Niche During Photoaging. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 2018 Dec 11 WB Mouse 1:10000 skin tissues

Citrus alkaline extracts prevent endoplasmic reticulum stress in type II alveolar epithelial cells to ameliorate pulmonary fibrosis via the ATF3/PINK1 pathway. PHYTOMEDICINE Phytomedicine. 2021 Aug;89:153599 WB Human 1 : 1000 Lung A549 cell

Label-free proteomic strategy to compare the proteome differences between longissimus lumborum and psoas major muscles during early postmortem periods. FOOD CHEMISTRY 2018 Jul 06 WB Bovine 1: 5000 M. longissimus lumborum (LL), M. psoas major (PM)

Modulation of TRIB3 and Macrophage Phenotype to Attenuate Insulin Resistance After Downhill Running in Mice. Frontiers in Physiology Front Physiol. 2021; 12: 637432 WB Mouse Adipose

Melatonin Improves the Fertilization Capacity of Sex-Sorted Bull Sperm by Inhibiting Apoptosis and Increasing Fertilization Capacitation via MT1. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Int J Mol Sci. 2019 Jan;20(16):3921 WB Bovine 1:5000 sperm

Heparanase from triple‑negative breast cancer and platelets acts as an enhancer of metastasis. INTERNATIONAL JOURNAL OF ONCOLOGY 2020 Aug 27 WB Human 1 : 10000 MDA-MB-231 cell

Stress aggravates and prolongs imiquimod‐induced psoriasis‐like epidermal hyperplasis and IL‐1β/IL‐23p40 production. JOURNAL OF LEUKOCYTE BIOLOGY J Leukocyte Biol. 2020 Jul;108(1):267-281 WB Mouse 1:10000 Skin

Citrus alkaline extracts prevent fibroblast senescence to ameliorate pulmonary fibrosis via activation of COX-2. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2019 Apr;112:108669 WB Mouse Primary murine lung fibroblasts (PMLFs)

Liver macrophages mediate effects of downhill running and caloric restriction on nonalcoholic fatty liver disease of high fat diet-fed mice. LIFE SCIENCES 2020 Jun 15 WB Mouse Liver

CD105 overexpression mediates drug-resistance in choriocarcinoma cells through BMP9/Smad pathway. Journal of Cancer J Cancer. 2020; 11(2): 272–283 WB Human 1:5000 JEG-3 cell

Acetyl-11-keto-β-boswellic acid inhibits the secretion of cytokines by dendritic cells via the TLR7/8 pathway in an imiquimod-induced psoriasis mouse model and in vitro. LIFE SCIENCES 2018 May 31 WB Mouse 1:5000 Skin BMDCs

Peptides derived from rice α-globulin reduce atherosclerosis in apolipoprotein E-deficient mice by inhibiting TNF-α-induced vascular endothelial cells injury. Journal of Functional Foods 2019 Sep 20 WB Human 1:5000 HUVECs

Substrate stiffness affects the morphology, proliferation, and radiosensitivity of cervical squamous carcinoma cells. TISSUE & CELL Tissue Cell. 2022 Feb;74:101681 WB，IF Human SiHa cell

Pollen developmental defects in ZD-CMS rice line explored by cytological, molecular and proteomic approaches. Journal of Proteomics 2014 May 28 WB Rice florets

Influence of the total saponin fraction from Dioscorea nipponica Makino on TLR2/4-IL1R receptor singnal pathway in rats of gouty arthritis. JOURNAL OF ETHNOPHARMACOLOGY J Ethnopharmacol. 2017 Jul;206:274 WB Rat 1: 5000 sunovial tissue

Paeonol ameliorates imiquimod-induced psoriasis-like skin lesions in BALB/c mice by inhibiting the maturation and activation of dendritic cells. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 2017 Mar 21 WB Mouse Skin

Relationship between coronary artery calcification and calcium deposition in the myocardium:. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH 2019 May 22 WB Human myocardial tissue

Qilin pills alleviate oligoasthenospermia by inhibiting Bax-caspase-9 apoptosis pathway in the testes of model rats. Oncotarget Oncotarget. 2018 Apr 24; 9(31): 21770–21782 WB Rat 1:5000 testis tissue

Jin, Yanling, et al. "Tissue factor potentiates adherence of breast cancer cells to human umbilical vein endothelial cells under static and flow conditions." Cell adhesion & migration 15.1 (2021): 74-83.

Wang, Zhichao, et al. "Citrus alkaline extract prevents endoplasmic reticulum stress in type II alveolar epithelial cells to ameliorate pulmonary fibrosis via the ATF3/PINK1 pathway." Phytomedicine (2021): 153599.

A Novel BCL-2 Inhibitor APG-2575 Exerts Synthetic Lethality With BTK or MDM2-p53 Inhibitor in Diffuse Large B-Cell Lymphoma. ONCOLOGY RESEARCH Oncol Res. 2020; 28(4): 331–344 WB Mouse OCI-LY8 xenograft tumors ,normal tissue SU-DHL 2 cell,SU-DHL 4 cell,OCI-LY 3 cell,OCI-LY 19 cell,OCI-LY 8 cell,OCI-LY 1 cell

Anti-Inflammation and Anti-Pyroptosis Activities of Mangiferin via Suppressing NF-κB/NLRP3/GSDMD Signaling Cascades INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Shidong Zhang WB Mouse

Ceramide Synthase 1 Inhibits Brain Metastasis of Non-Small Cell Lung Cancer by Interacting with USP14 and Downregulating the PI3K/AKT/mTOR Signaling Pathway Cancers Hongru Li WB Human benign prostatic hyperplasia (BPH) tissue H1299 cell,PC-9 cell

The SLITRK4-CNPY3 axis promotes liver metastasis of gastric cancer by enhancing the endocytosis and recycling of TrkB in tumour cells CELLULAR ONCOLOGY Li Jun WB Human BGC823 cell,MKN28 cell

MAOA suppresses the growth of gastric cancer by interacting with NDRG1 and regulating the Warburg effect through the PI3K/AKT/mTOR pathway. Li Jun WB Human 1:5000 BGC-823 cell,HGC-27 cell

Regulation of secondary hair follicle cycle in cashmere goats by miR-877-3p targeting IGFBP5 gene. JOURNAL OF ANIMAL SCIENCE Xuefeng Fu WB Goat 1:10000 dermal papilla cell

ERRα promotes glycolytic metabolism and targets the NLRP3/caspase-1/GSDMD pathway to regulate pyroptosis in endometrial cancer. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH Pengming Sun WB Human 1:2000 KLE cell，HEC-1A cell

Downhill running and caloric restriction attenuate insulin resistance associated skeletal muscle atrophy via the promotion of M2-like macrophages through TRIB3-AKT pathway FREE RADICAL BIOLOGY AND MEDICINE Wei Luo WB Mouse 1:5000 skeletal muscle

Diet Change Improves Obesity and Lipid Deposition in High-Fat Diet-Induced Mice Nutrients Tengteng Ji WB Mouse 1:10000 liver tissue,adipose tissue

Novel sulfonyl-substituted tetrandrine derivatives for colon cancer treatment by inducing mitochondrial apoptosis and inhibiting PI3K/AKT/mTOR pathway BIOORGANIC CHEMISTRY Jie Ling WB Human,Mouse HCT116 cell,CT26 cell

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs