• Target：
• XRCC4

• Fields：
• >>Non-homologous end-joining

• Gene Name：
• XRCC4

• Protein Name：
• DNA repair protein XRCC4

• Human Gene Id：
• 7518

• Human Swiss Prot No：
• Q13426

• Mouse Gene Id：
• 108138

• Mouse Swiss Prot No：
• Q924T3

• Immunogen：
• Synthetic Peptide of XRCC4

• Specificity：
• The antibody detects endogenous XRCC4 proteins.

• Formulation：
• PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:2000 IP:1:200 IF 1:200 IHC 1:50-300

• Purification：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• XRCC4;DNA repair protein XRCC4;X-ray repair cross-complementing protein 4

• Observed Band(KD)：
• 38kD

• Background：
• The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants. [provided by RefSeq, Dec 2015],

• Function：
• function:Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.,PTM:Monoubiquitinated.,PTM:Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.,PTM:Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.,similarity:Belongs to the XRCC4 family.,subunit:Homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dep

• Subcellular Location：
• Nucleus . Chromosome . Localizes to site of double-strand breaks. .; [Protein XRCC4, C-terminus]: Cytoplasm . Translocates from the nucleus to the cytoplasm following cleavage by caspase-3 (CASP3). .

• Expression：
• Widely expressed.

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