GFP-Tag Monoclonal Antibody(Mix)
- Catalog No.:YM3124
- Applications:WB;IF;IP;CoIP
- Reactivity:Species independent
- Target:
- GFP-Tag
- Gene Name:
- GFP-Tag
- Protein Name:
- GFP Tag
- Immunogen:
- Recombinant Protein of GFP
- Specificity:
- GFP antibody is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.
- Formulation:
- PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
- Source:
- Monoclonal, Mouse
- Dilution:
- WB 1:5000 IP: 1:200. IF 1:50-200
- Purification:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Background:
- Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
MicroRNA-10 Family Promotes the Epithelial-to-Mesenchymal Transition in Renal Fibrosis by the PTEN/Akt Pathway CURRENT ISSUES IN MOLECULAR BIOLOGY Chaokun Wang, Yichen Shuai, Chuan Zhao, Fengrui Yang, Weilian Su, Zhifen Ning, Guoxia Li WB Human HK-2 cell
Inhibition of N-Acetyltransferase 10 Suppresses the Progression of Prostate Cancer through Regulation of DNA Replication IP Human 1:2000 /VCaP cells, PC-3 cells
Disrupting PHF8-TOPBP1 connection elicits a breast tumor-specific vulnerability to chemotherapeutics Cancer Lett. 2022 Apr;530:29. WB,IP Human
A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis. Theranostics Theranostics. 2020; 10(23): 10769–10790 WB,IP Human MCF-7 cell,LacO cell
Formation of Kiss1R/GPER Heterocomplexes Negatively Regulates Kiss1R-mediated Signalling through Limiting Receptor Cell Surface Expression. JOURNAL OF MOLECULAR BIOLOGY J Mol Biol. 2021 Apr;433:166843 WB,IP Human HEK293 cell
The NAD-dependent deacetylase SIRT2 regulates T cell differentiation involved in tumor immune response. International Journal of Biological Sciences Int J Biol Sci. 2020; 16(15): 3075–3084 WB Human 1:1000 CD8+ T cells,HEK293T cell
Zhao, Xiumei, et al. "Ruanjian Sanjie Decoction Induces Apoptosis in Triple-negative Breast Cancer Cells by Downregulating cIAP1/2 and XIAP." (2020).
AtHD2D, A Plant-specific Histone Deacetylase Involved in Abscisic Acid Response and Lateral Root Development JOURNAL OF EXPERIMENTAL BOTANY Zhaofen Han CoIP Arabidopsis thaliana
SPINDLY interacts with EIN2 to facilitate ethylene signaling-mediated fruit ripening in tomato
SEC1-C3H39 module fine-tunes cold tolerance by mediating its target mRNAs degradation in tomato
Cooperative effects of SIRT1 and SIRT2 on APP acetylation. AGING CELL Liu Cao IF Human 1:1000 HEK293 cell
Antagonistic MADS-box transcription factors SEEDSTICK and SEPALLATA3 form a transcriptional regulatory network that regulates seed oil accumulation Journal of Integrative Plant Biology Shuangcheng He CoIP,WB Arabidopsis thaliana siliques
Epstein-Barr virus suppresses N6-Methyladenosine modification of TLR9 to promote immune evasion JOURNAL OF BIOLOGICAL CHEMISTRY Xiaoyue Zhang IP,WB Human HEK293 cell
BnaC06.WIP2-BnaA09.STM transcriptional regulatory module promotes leaf lobe formation in Brassica napus INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Shuangcheng He CoIP Brassica napus Leaf
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- Antibody-FAQs
- Products Images
- ADAR1 links R-loop homeostasis to ATR activation in replication stress response. NUCLEIC ACIDS RESEARCH Lei Shi WB,CoIP Human HeLa cell
- Cooperative effects of SIRT1 and SIRT2 on APP acetylation. AGING CELL Liu Cao IF Human 1:1000 HEK293 cell
- Western blot analysis of GFP transfected Hela, diluted at 1) 1:5000 2) 1:10000
- 1) Input (control) 2) IP products, antibody dilution 1:200