p21 (PTR2559) Mouse mAb
- Catalog No.:YM3802
- Applications:WB;IHC
- Reactivity:Human;Mouse
- Target:
- P21
- Gene Name:
- CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1
- Protein Name:
- p21
- Human Gene Id:
- 1026
- Human Swiss Prot No:
- P38936
- Mouse Gene Id:
- 12575
- Mouse Swiss Prot No:
- P39689
- Immunogen:
- Synthesized peptide derived from human p21 AA range: 1-100
- Specificity:
- This antibody detects endogenous levels of p21 at Human
- Formulation:
- Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
- Source:
- Monoclonal, Mouse IgG1, Kappa
- Dilution:
- WB 1:500-2000 IHC 1:50-200
- Purification:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- Cyclin-dependent kinase inhibitor 1 ;CDK-interacting protein 1;Melanoma differentiation-associated protein 6;MDA-6;p21;
- Observed Band(KD):
- 21kD
- Function:
- function:May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.,induction:By p53, mezerein (antileukemic compound) and interferon beta.,PTM:Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.,similarity:Belongs to the CDI family.,tissue specificity:Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.,
- Subcellular Location:
- Cytoplasm . Nucleus .
- Expression:
- Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
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- Antibody-FAQs
- Products Images
- Whole cell lysates of A549 were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p21(PTR2559) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Predicted band size: 21kDa Observed band size: 21kDa
- Whole cell lysates of HEK293 were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p21(PTR2559) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Predicted band size: 21kDa Observed band size: 21kDa
- Whole cell lysates of MCF7 were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p21(PTR2559) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: MCF7 Predicted band size: 21kDa Observed band size: 21kDa