p21 (PTR2559) mouse mAb

    • Catalog No.:YM3802
    • Applications:IHC;WB;IF;ELISA
    • Reactivity:Human;Mouse;
      • Target:
      • P21
      • Gene Name:
      • CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1
      • Protein Name:
      • p21
      • Human Gene Id:
      • 1026
      • Human Swiss Prot No:
      • P38936
      • Mouse Swiss Prot No:
      • P39689
      • Immunogen:
      • Synthesized peptide derived from human p21 AA range: 1-100
      • Specificity:
      • This antibody detects endogenous levels of p21 protein.
      • Formulation:
      • PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
      • Source:
      • Mouse, Monoclonal/IgG1, kappa
      • Dilution:
      • IHC 1:200-1000. WB 1:500-2000. IF 1:100-500. ELISA 1:1000-5000
      • Purification:
      • Protein G
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • Cyclin-dependent kinase inhibitor 1 ;CDK-interacting protein 1;Melanoma differentiation-associated protein 6;MDA-6;p21;
      • Molecular Weight(Da):
      • 21kD
      • Observed Band(KD):
      • 21kD
      • Function:
      • function:May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.,induction:By p53, mezerein (antileukemic compound) and interferon beta.,PTM:Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.,similarity:Belongs to the CDI family.,tissue specificity:Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.,
      • Subcellular Location:
      • Cytoplasmic, Nuclear
      • Expression:
      • Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
      • Products Images
      • Whole cell lysates of A549 were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p21(PTR2559) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: A549
      • Whole cell lysates of HEK293 were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p21(PTR2559) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293
      • Whole cell lysates of MCF7 were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p21(PTR2559) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: MCF7