• Target：
• Insulin

• Fields：
• >>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cGMP-PKG signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Phospholipase D signaling pathway;>>Oocyte meiosis;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Regulation of actin cytoskeleton;>>Insulin signaling pathway;>>Insulin secretion;>>Ovarian steroidogenesis;>>Progesterone-mediated oocyte maturation;>>Prolactin signaling pathway;>>Regulation of lipolysis in adipocytes;>>Type II diabetes mellitus;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Type I diabetes mellitus;>>Maturity onset diabetes of the young;>>Aldosterone-regulated sodium reabsorption;>>Alzheimer disease;>>Prostate cancer;>>Diabetic cardiomyopathy

• Gene Name：
• INS

• Protein Name：
• Insulin [Cleaved into: Insulin B chain; Insulin A chain]

• Human Gene Id：
• 3630

• Human Swiss Prot No：
• P01308

• Immunogen：
• Synthesized peptide derived from human Insulin AA range: 25-110

• Specificity：
• The antibody can specifically recognize human Insulin protein.

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Mouse, Monoclonal/IgG2b, kappa

• Dilution：
• IHC 1:200-400. ELISA 1:500-5000

• Purification：
• The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Molecular Weight(Da)：
• 12kD

• Observed Band(KD)：
• 9kD

• Background：
• After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010],

• Function：
• disease:Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].,function:Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.,function:Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.,function:The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.,mass spectrometry: PubMed:12586351; PubMed:15359740,online information:Clinical information on Eli Lilly insu

• Subcellular Location：
• Cytoplasmic

• Expression：
• Blood,Liver,Muscle,Pancreas,

Study on the mechanism of modified Gegen Qinlian decoction in regulating the intestinal flora-bile acid-TGR5 axis for the treatment of type 2 diabetes mellitus based on macro genome sequencing and targeted metabonomics integration PHYTOMEDICINE Rong Liu IHC Mouse pancreas tissue

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs