p90RSK (phospho Thr359/Ser363) (PT0332R) PT® Rabbit mAb
- Catalog No.:YM8196
- Applications:WB;IHC;IF;IP;ELISA
- Reactivity:Human; Mouse; Rat;
- Target:
- Rsk-1
- Fields:
- >>MAPK signaling pathway;>>Oocyte meiosis;>>mTOR signaling pathway;>>Thermogenesis;>>Long-term potentiation;>>Neurotrophin signaling pathway;>>Progesterone-mediated oocyte maturation;>>Insulin resistance;>>Yersinia infection;>>Chemical carcinogenesis - receptor activation
- Gene Name:
- RPS6KA1
- Protein Name:
- Ribosomal protein S6 kinase alpha-1
- Human Gene Id:
- 6195
- Human Swiss Prot No:
- Q15418
- Mouse Swiss Prot No:
- P18653
- Rat Gene Id:
- 81771
- Rat Swiss Prot No:
- Q63531
- Specificity:
- endogenous
- Formulation:
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- Source:
- Monoclonal, rabbit, IgG, Kappa
- Dilution:
- IHC 1:500-1:2000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;
- Purification:
- Protein A
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- RPS6KA1;MAPKAPK1A;RSK1;Ribosomal protein S6 kinase alpha-1;S6K-alpha-1;90 kDa ribosomal protein S6 kinase 1;p90-RSK 1;p90RSK1;p90S6K;MAP kinase-activated protein kinase 1a;MAPK-activated protein kinase 1a;MAPKAP kinase 1a;MAPKAP
- Molecular Weight(Da):
- 83kD
- Observed Band(KD):
- 83kD
- Background:
- ribosomal protein S6 kinase A1(RPS6KA1) Homo sapiens This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],
- Function:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,cofactor:Magnesium.,enzyme regulation:Activated by multiple phosphorylations on threonine and serine residues.,function:Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.,PTM:Autophosphorylated on Ser-380, as part of the activation process.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 2 protein kinase domains.,subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells. Transiently dissociates following mitogenic s
- Subcellular Location:
- Cytoplasm, Nucleus
- Expression:
- Colon,Epithelium,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-p90RSK (phospho Thr359/Ser363) (PT0067R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: NIH-3T3 Lane 3: PC-12 Predicted band size: 83kDa Observed band size: 83kDa
- Human colon carcinoma was stained with anti-p90RSK (phospho Thr359/Ser363) rabbit antibody
- Mouse colon was stained with anti-p90RSK (phospho Thr359/Ser363) rabbit antibody
- Rat colon was stained with anti-p90RSK (phospho Thr359/Ser363) rabbit antibody