Rsk-1 (phospho Ser380) Polyclonal Antibody
- Catalog No.：YP0314
- Applications：WB,IHC-p,IF(paraffin section),ELISA
- Reactivity：Human,Mouse,Rat
- Gene Name：
- RPS6KA1
- Protein Name：
- Ribosomal protein S6 kinase alpha-1
- Human Gene Id：
- 6195
- Human Swiss Prot No：
- Q15418
- Mouse Swiss Prot No：
- P18653
- Rat Gene Id：
- 81771
- Rat Swiss Prot No：
- Q63531
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human p90 RSK around the phosphorylation site of Ser380. AA range:346-395
- Specificity：
- Phospho-Rsk-1 (S380) Polyclonal Antibody detects endogenous levels of Rsk-1 protein only when phosphorylated at S380.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit
- Dilution：
- Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -20°C/1 year
- Other Name：
- RPS6KA1; MAPKAPK1A; RSK1; Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAP
- Molecular Weight(Da)：
- 82723
- Observed Band(KD)：
- 83
- Background：
- ribosomal protein S6 kinase A1(RPS6KA1) Homo sapiens This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],
- Function：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,cofactor:Magnesium.,enzyme regulation:Activated by multiple phosphorylations on threonine and serine residues.,function:Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.,PTM:Autophosphorylated on Ser-380, as part of the activation process.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 2 protein kinase domains.,subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells. Transiently dissociates following mitogenic s
- Subcellular Location：
- nucleus,nucleoplasm,cytoplasm,cytosol,
- Expression：
- Colon,Epithelium,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of 293 cells using Phospho-Rsk-1 (S380) Polyclonal Antibody
- Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p90 RSK (Phospho-Ser380) Antibody
- Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using p90 RSK (Phospho-Ser380) Antibody. The lane on the right is blocked with the phospho peptide.
