• Target：
• NFkB p65

• Fields：
• >>Antifolate resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>NF-kappa B signaling pathway;>>HIF-1 signaling pathway;>>Sphingolipid signaling pathway;>>Mitophagy - animal;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Longevity regulating pathway;>>Cellular senescence;>>Osteoclast differentiation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>Cytosolic DNA-sensing pathway;>>C-type lectin receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>TNF signaling pathway;>>Neurotrophin signaling pathway;>>Prolactin signaling pathway;>>Adipocytokine signaling pathway;>>Relaxin signaling pathway;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>AGE-RAGE signaling pathway in diabe

• Gene Name：
• RELA

• Protein Name：
• Transcription factor p65

• Human Gene Id：
• 5970

• Human Swiss Prot No：
• Q04206

• Mouse Gene Id：
• 19697

• Mouse Swiss Prot No：
• Q04207

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:1000-1:4000,WB 1:1000-1:5000,IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200,

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• RELA;NFKB3;Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

• Molecular Weight(Da)：
• 65kD

• Observed Band(KD)：
• 70kD

• Background：
• NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

• Function：
• function:NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by in

• Subcellular Location：
• Cytoplasm, Nucleus

• Expression：
• Bone,Colon,Pancreas,Placenta,

Radiation-induced bystander effect on the genome of bone marrow mesenchymal stem cells in lung cancer

Tyrosol inhibits NF-κB pathway in the treatment of enterotoxigenic Escherichia coli-induced diarrhea in mice MICROBIAL PATHOGENESIS Fazheng Yu, Jian Guo, Hong lin Ren, Shiying Lu, Zhaoqi He, Jiang Chang, Xueyu Hu, Ruoran Shi, Yuanyuan Jin, Yansong Li, Zengshan Liu, Xiaoxu Wang, Pan Hu WB Mouse colonic tissue

Role of salivary glycopatterns for oral microbiota associated with gastric cancer. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES2022 Jun;209:1368. Mouse RAW264.7 cell

Mechanical loading mitigates osteoarthritis symptoms by regulating the inflammatory microenvironment in a mouse model 2022 Feb 27. WB Mouse articular cartilage

Chronic glucocorticoids exposure enhances neurodegeneration in the frontal cortex and hippocampus via NLRP-1 inflammasome activation in male mice. BRAIN BEHAVIOR AND IMMUNITY Brain Behav Immun. 2016 Feb;52:58 WB Mouse frontal cortex , hippocampus

Study on the Chemical Constituents and Anti-Migraine Activity of Supercritical CO2 Extracts of Zanthoxylum schinifolium.. Frontiers in Pharmacology Front Pharmacol. 2021 Nov;12:744035-744035 WB Rat Brain

Novel peptide GX1 inhibits angiogenesis by specifically binding to transglutaminase-2 in the tumorous endothelial cells of gastric cancer. Cell Death & Disease Cell Death Dis. 2018 May;9(6):1-16 WB Human 1:500 HUVECs,SGC7901 cell,GES cell

Dibromoacetic Acid Induced Hepatotoxicity in Mice through Oxidative Stress and Toll-Like Receptor 4 Signaling Pathway Activation. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2019;2019:5637235 WB Mouse Liver

Synergistic suppression effect on tumor growth of acute myeloid leukemia by combining cytarabine with an engineered oncolytic vaccinia virus. OncoTargets and Therapy Oncotargets Ther. 2018; 11: 6887–6900 WB Human 1:1000 Leukemia cell

STC1 promotes cell apoptosis via NF-κB phospho-P65 Ser536 in cervical cancer cells. Oncotarget Oncotarget. 2017 Jul 11; 8(28): 46249–46261 WB Human 1:100 cervical cancer tissue

Effects on IL-1β signaling activation induced by water and organic extracts of fine particulate matter (PM2.5) in vitro. ENVIRONMENTAL POLLUTION 2018 Mar 15 WB Mouse 1:2000 RAW264.7 cell

CD38 enhances the proliferation and inhibits the apoptosis of cervical cancer cells by affecting the mitochondria functions. MOLECULAR CARCINOGENESIS Mol Carcinogen. 2017 Oct;56(10):2245-2257 WB Human,Mouse 1:1000 CaSki cell-xenograft,cervical cancer tissues CaSki cell， HeLa cell

Renin-angiotensin system activation accelerates atherosclerosis in experimental renal failure by promoting endoplasmic reticulum stress-related inflammation. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Int J Mol Med. 2017 Mar;39(3):613-621 WB Mouse 1:500 aorta tissue RAW264.7 macrophages

CD137 Regulates NFATc1 Expression in Mouse VSMCs through TRAF6/NF-κB p65 Signaling Pathway. MEDIATORS OF INFLAMMATION Mediat Inflamm. 2015;2015:639780 WB Mouse 1 : 1000 VSMCs

Berberine hydrochloride attenuates lipopolysaccharide-induced endometritis in mice by suppressing activation of NF-κB signal pathway. INTERNATIONAL IMMUNOPHARMACOLOGY 2014 Nov 15 WB Mouse uteri

Oat fiber supplementation alleviates intestinal inflammation and ameliorates intestinal mucosal barrier via acting on gut microbiota-derived metabolites in LDLR–/– mice. NUTRITION Nutrition. 2022 Mar;95:111558 WB Mouse Aortic tissue，intestinal tissue

Lactoferrin suppresses lipopolysaccharide-induced endometritis in mice via down-regulation of the NF-κB pathway. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2015 Sep;28:695 WB Mouse 1:1000 uterine tissues

Cold-inducible RNA-binding protein (CIRBP) promotes porcine reproductive and respiratory syndrome virus (PRRSV)-induced inflammatory response. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2020 Sep;86:106728 WB Porcine 1:1000 3D4/21 cell

Rhein protects against barrier disruption and inhibits inflammation in intestinal epithelial cells. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2019 Jun;71:321 WB Rat IEC-6 cell

LXA4 ameliorates cerebrovascular endothelial dysfunction by reducing acute inflammation after subarachnoid hemorrhage in rats. NEUROSCIENCE Neuroscience. 2019 Jun;408:105 WB Rat 1:1000 brain

Fra-1 Inhibits Cell Growth and the Warburg Effect in Cervical Cancer Cells via STAT1 Regulation of the p53 Signaling Pathway. Frontiers in Cell and Developmental Biology Front Cell Dev Biol. 2020 Sep;0:1022 WB Human 1 : 1000 HeLa cell

N′-[(3-[benzyloxy]benzylidene]-3,4,5-trihydroxybenzohydrazide (1) protects mice against colitis induced by dextran sulfate sodium through inhibiting NFκB/IL-6/STAT3 pathway. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Biochem Bioph Res Co. 2016 Aug;477:290 WB Mouse colon

Allyl Sulfide Counteracts 1-Bromopropane-Induced Neurotoxicity by Inhibiting Neuroinflammation and Oxidative Stress. TOXICOLOGICAL SCIENCES 2019 Feb 01 WB Rat cortex

The antitumor effect of hinesol, extract from Atractylodes lancea (Thunb.) DC. by proliferation, inhibition, and apoptosis induction via MEK/ERK and NF‐κB pathway in non–small cell lung cancer cell lines A549 and NCI‐H1299. JOURNAL OF CELLULAR BIOCHEMISTRY 2019 Jul 24 WB Human A549 cell

Exogenous ghrelin ameliorates acute lung injury by modulating the nuclear factor κB inhibitor kinase/nuclear factor κB inhibitor/nuclear factor κB pathway after hemorrhagic shock. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2019 Apr;69:95 WB Rat 1:1000 lung tissue

The receptor for activated protein kinase C promotes cell growth, invasion and migration in cervical cancer. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2017 Nov;51(5):1497-1507 WB Human 1:1000 cervical cancer tissues CaSki cell

The Antitumor Effect of Suicide Gene Therapy Using Bifidobacterium infantis–mediated Herpes Simplex Virus Thymidine Kinase/Ganciclovir in a Nude Mice Model of Renal Cell Carcinoma. UROLOGY 2014 Aug 12 WB Mouse 1:500 786-O cell-Xenograft

Chronic dexamethasone treatment results in hippocampal neurons injury due to activate NLRP1 inflammasome in vitro. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2017 Aug;49:222 WB Rat hippocampus neurons

p21-activated kinase 4 as a switch between caspase-8 apoptosis and NF-κB survival signals in response to TNF-α in hepatocarcinoma cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2018 Aug 24 WB Human SK-Hep1 cell, HepG2 cell

Dopamine receptor D2 inhibition alleviates diabetic hepatic stellate cells fibrosis by regulating the TGF‐β1/Smads and NFκB pathways. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY Clin Exp Pharmacol P. 2021 Mar;48(3):370-380 WB Rat 1 : 1000 Liver hepatic stellate cells (HSCs)

Guiqi Baizhu Decoction Alleviates Radiation Inflammation in Rats by Modulating the Composition of the Gut Microbiota. Evidence-based Complementary and Alternative Medicine 2020 Oct 20 WB Rat Colon

Safety profile of the transcription factor EB (TFEB)-based gene therapy through intracranial injection in mice. Translational Neuroscience Transl Neurosci. 2020 Jan;11(1):241-250 WB Mouse brain tissue

Lu, Qian, et al. "Norisoboldine suppresses VEGF-induced endothelial cell migration via the cAMP-PKA-NF-κB/Notch1 pathway." PloS one 8.12 (2013): e81220.

N′-[(3-[benzyloxy] benzylidene]-3, 4, 5-trihydroxybenzohydrazide (1) protects mice against colitis induced by dextran sulfate sodium through inhibiting NFκB/IL-6/STAT3 pathway." Biochemical and biophysical research communications 477.2 (2016): 290-296.

The receptor for activated protein kinase C promotes cell growth, invasion and migration in cervical cancer. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2017 Nov;51(5):1497-1507 WB Human 1:1000 cervical cancer tissues CaSki cell

Correlation of In Vivo and In Vitro Assay Results for Assessment of Free Radical Scavenging Activity of Green Tea Nutraceuticals. JOURNAL OF FOOD SCIENCE J Food Sci. 2016 Jul;81(7):C1707-C1715 ELISA Rat hepatic

LXA4 ameliorates cerebrovascular endothelial dysfunction by reducing acute inflammation after subarachnoid hemorrhage in rats. NEUROSCIENCE Neuroscience. 2019 Jun;408:105 WB Rat 1:1000 brain

Protective effect of astaxanthin on tuberculosis-associated inflammatory lung injury EXPERIMENTAL BIOLOGY AND MEDICINE Xiaoguang Zou WB Mouse MLE-12 cells

Hydrogen gas ameliorates acute alcoholic liver injury via anti-inflammatory and antioxidant effects and regulation of intestinal microbiota. Weiping Fan WB Mouse 1:1000 liver tissue

Hypoxic preconditioned mesenchymal stem cells ameliorate rat brain injury after cardiopulmonary resuscitation by suppressing neuronal pyroptosis. Feng Chen WB Rat cortical tissue

Dangshen Huangjiu exerts prevention of gastric mucosal injury and inhibits Akt/NF-κB signaling. Fangdi Hu WB Rat,Human gastric mucosal tissues GES-1 cell

ω3-PUFA alleviates neuroinflammation by upregulating miR-107 targeting PIEZO1/NFκB p65 INTERNATIONAL IMMUNOPHARMACOLOGY Hailin Liu WB Mouse 1:1000 hippocampus tissue 293 T cell

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