IKK α (PT0501R) PT® Rabbit mAb

    • Catalog No.:YM8329
    • Applications:WB;IHC;IF;IP;ELISA
    • Reactivity:Human; Mouse; Rat;
      • Target:
      • IKKα
      • Fields:
      • >>Antifolate resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>Chemokine signaling pathway;>>NF-kappa B signaling pathway;>>FoxO signaling pathway;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Osteoclast differentiation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>Cytosolic DNA-sensing pathway;>>C-type lectin receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>TNF signaling pathway;>>Adipocytokine signaling pathway;>>Alcoholic liver disease;>>Alzheimer disease;>>Epithelial cell signaling in Helicobacter pylori infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Yersinia infection;>>Chagas disease;>>Toxoplasmosis;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Human pap
      • Gene Name:
      • CHUK
      • Protein Name:
      • Inhibitor of nuclear factor kappa-B kinase subunit alpha
      • Human Gene Id:
      • 1147
      • Human Swiss Prot No:
      • O15111
      • Mouse Swiss Prot No:
      • Q60680
      • Specificity:
      • endogenous
      • Formulation:
      • PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
      • Source:
      • Monoclonal, rabbit, IgG, Kappa
      • Dilution:
      • IHC 1:100-1:500;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;
      • Purification:
      • Protein A
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • CHUK;IKKA;TCF16;Inhibitor of nuclear factor kappa-B kinase subunit alpha;I-kappa-B kinase alpha;IKK-A;IKK-alpha;IkBKA;IkappaB kinase;Conserved helix-loop-helix ubiquitous kinase;I-kappa-B kinase 1;IKK1;Nuclear factor NF-kappa-B
      • Molecular Weight(Da):
      • 85kD
      • Observed Band(KD):
      • 85kD
      • Background:
      • This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].,enzyme regulation:Activated when phosphorylated and inactivated when dephosphorylated.,function:Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.,PTM:Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK
      • Subcellular Location:
      • Cytoplasm, Nucleus
      • Expression:
      • Widely expressed.
      • Products Images
      • Rat kidney was stained with anti-IKK α (PT0501R) rabbit antibody
      • Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IKK α (PT0501R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: K562 Lane 3: 3T3-L1 Predicted band size: 85kDa Observed band size: 85kDa
      • Human breast carcinoma was stained with anti-IKK α (PT0501R) rabbit antibody
      • Mouse kidney was stained with anti-IKK α (PT0501R) rabbit antibody