p21 (PT0544R) PT® Rabbit mAb
- Catalog No.:YM8364
- Applications:WB;IHC;IF;IP;ELISA
- Reactivity:Mouse; Rat;
- Target:
- P21
- Gene Name:
- CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1
- Protein Name:
- p21
- Human Gene Id:
- 1026
- Human Swiss Prot No:
- P38936
- Mouse Gene Id:
- 12575
- Mouse Swiss Prot No:
- P39689
- Specificity:
- endogenous
- Formulation:
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- Source:
- Monoclonal, rabbit, IgG, Kappa
- Dilution:
- IHC 1:500-1:2000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;
- Purification:
- Protein A
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- Cyclin-dependent kinase inhibitor 1 ;CDK-interacting protein 1;Melanoma differentiation-associated protein 6;MDA-6;p21;
- Molecular Weight(Da):
- 18kD
- Observed Band(KD):
- 18kD
- Background:
- This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lac
- Function:
- function:May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.,induction:By p53, mezerein (antileukemic compound) and interferon beta.,PTM:Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.,similarity:Belongs to the CDI family.,tissue specificity:Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.,
- Subcellular Location:
- Nucleus
- Expression:
- Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
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- Antibody-FAQs
- Products Images
- Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-p21 (PT0544R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat womb Lane 2: RAW264.7 Predicted band size: 18kDa Observed band size: 18kDa
- Rat skin was stained with anti-p21 (PT0544R) rabbit antibody